| Zugriffsnummer | 55922 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | CCQM-K177 - Determination of growth hormone in serum using isotope dilution mass spectrometry - final report |
| Autor(in); Institution |
Römmert, Anne-Katrin; 3.2, Biochemie, PTB-Braunschweig
Ohlendorf, Rüdiger; 3.2, Biochemie, PTB-Braunschweig
Rui, Zhai; National Institute of Metrology (NIM), Beijing, CHINA
Zhanying, Chu; National Institute of Metrology (NIM), Beijing, CHINA
YouXun, Jin; National Institute of Metrology (NIM), Beijing, CHINA
Liqing, Wu; National Institute of Metrology (NIM), Beijing, CHINA
Xinhua, Dai; National Institute of Metrology (NIM), Beijing, CHINA
Lixing, Yu; National Institute of Metrology (NIM), Beijing, CHINA
L.H., Tong; Government Laboratory, HKSAR, Ho Man Tin, HONG KONG
Qinde, Liu; Health Sciences Authority (HSA), Singapore, SINGAPORE
Tomoya, Kinumi; National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), JAPAN
Bas, Sleumer; University Medical Center Groningen (UMCG), Groningen, THE NETHERLANDS
Michael, Vos; University Medical Center Groningen (UMCG), Groningen, THE NETHERLANDS
|
| Quelle/Jahr | Metrologia: 62 (2025), 1A, 1 - 69 |
| Artikelnummer | 08026 |
| ISSN | 0026-1394 (print) ; 1681-7575 (online) |
| DOI | |
| Verlag | Bristol: IOP Publishing |
| Freie Schlagworte | Growth hormone ; isotope-dilution mass spectrometry (ID-MS) ; serum ; LC-MS/MS; key comparison |
| Zusammenfassung | This comparative measurement aims to demonstrate the ability to determine the amount of substance of a small protein in a biological matrix such as blood serum in the concentration range around 0.50 pmol/g (0.23 pmol/g to 0.90 pmol/g) using isotope dilution mass spectrometry. Growth Hormone (GH) was selected as the model protein, with the measurand being total GH in serum. A pooled serum sample from blood donors was used as study material. Five National Metrology Institutes (NMIs) participated and submitted results, all of which were included in the calculation of the key comparison reference value. All participants agreed to use the DerSimonian and Laird method to establish the consensus value. Due to greater-than-expected variability among participants compared to the uncertainties reported by their laboratories, an excess measurement uncertainty (referred to as "dark uncertainty") was estimated. This value provided a quantitative assessment of previously unidentified uncertainty components. The DerSimonian and Laird consensus value was determined to be 11.79 ± 0.99 ng/g, with the dark uncertainty (τ) estimated at 2.09 ng/g. |
| Kostenfreier Zugang | Freier Zugang |
| Themenbereich der Metrologie | Metrologie in der Chemie und Stoffeigenschaften |
Zitierung
Arsene, C., Römmert, A.-K., Ohlendorf, R., Rui, Z., Zhanying, C., YouXun, J., Liqing, W., Xinhua, D., Lixing, Y., L.H., T., Qinde, L., Tomoya, K., Bas, S., & Michael, V. (2025). CCQM-K177 - Determination of growth hormone in serum using isotope dilution mass spectrometry - final report. Metrologia, 62(1A), 1–69. https://doi.org/10.1088/0026-1394/62/1A/08026