| Zugriffsnummer | 55348 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | Synergistic effects of UVB and ionizing radiation on human non-malignant cells: Implications for ozone depletion and secondary cosmic radiation exposure |
| Autor(in); Institution |
Gkikoudi, Angeliki; National Technical University of Athens, School of Applied Mathematical and Physical Sciences, Physics Department, DNA Damage Laboratory, Athens, GREECE; National Centre for Scientific Research “Demokritos”, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, Laboratory of Health Physics, Radiobiology & Cytogenetics, Athens, GREECE
Manda, Gina; Victor Babes National Institute of Pathology, Radiobiology Laboratory, Bucharest, ROMANIA
Beinke, Christina; University of Ulm, Bundeswehr Institute of Radiobiology, Munich, GERMANY
Dragnea, Elena-Mihaela; Victor Babes National Institute of Pathology, Radiobiology Laboratory, Bucharest, ROMANIA
Dobre, Maria; Victor Babes National Institute of Pathology, Radiobiology Laboratory, Bucharest, ROMANIA
Neagoe, Ionela Victoria; Victor Babes National Institute of Pathology, Radiobiology Laboratory, Bucharest, ROMANIA
Sangsuwan, Traimate; University of Caen Normandy, Caen, ABTE/ToxEMAC Laboratory, Caen, FRANCE; Stockholm University, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm, SWEDEN
Haghdoost, Siamak; University of Caen Normandy, Caen, ABTE/ToxEMAC Laboratory, Caen, FRANCE; Stockholm University, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm, SWEDEN
Vasilopoulos, Spyridon N.; National Technical University of Athens, School of Applied Mathematical and Physical Sciences, Physics Department, DNA Damage Laboratory, Athens, GREECE
Triantopoulou, Sotiria; National Centre for Scientific Research “Demokritos”, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, Laboratory of Health Physics, Radiobiology & Cytogenetics, Athens, GREECE
Georgakopoulou, Anna; National Technical University of Athens, School of Applied Mathematical and Physical Sciences, Physics Department, DNA Damage Laboratory, Athens, GREECE
Tremi, Ioanna; National Technical University of Athens, School of Applied Mathematical and Physical Sciences, Physics Department, DNA Damage Laboratory, Athens, GREECE; National and Kapodistrian University of Athens, Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, Athens, GREECE
Koutsoudaki, Paraskevi N.; National and Kapodistrian University of Athens, Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, Athens, GREECE
Havaki, Sophia; National and Kapodistrian University of Athens, Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, Athens, GREECE
Gorgoulis, Vassilis G.; National and Kapodistrian University of Athens, Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, Athens, GREECE; Academy of Athens, Biomedical Research Foundation, Athens, GREECE; University of Dundee, Ninewells Hospital and Medical School, Dundee, UK
Kokkoris, Michael; National Technical University of Athens, Group of Nuclear Physics, Physics Department, School of Applied Mathematical and Physical Sciences, Zografou, GREECE
Krasniqi, Faton; 6.3, Strahlenschutzdosimetrie, PTB-Braunschweig
Terzoudi, Georgia I.; National Centre for Scientific Research “Demokritos”, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, Laboratory of Health Physics, Radiobiology & Cytogenetics, Athens, GREECE
Georgakilas, Alexandros G.; National Technical University of Athens, School of Applied Mathematical and Physical Sciences, Physics Department, DNA Damage Laboratory, Athens, GREECE
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| Quelle/Jahr | Biomolecules: 15 (2025), 4 |
| Artikelnummer | 536 |
| Availability | [online only] |
| ISSN | 2218-273X (online) |
| DOI | |
| Verlag | Basel: MDPI |
| Freie Schlagworte | solar UV radiation ; fibroblasts ; DNA damage ; keratinocytes ; monocytes ; biological effects ; secondary cosmic radiation ; ozone ; UVB |
| Zusammenfassung | The ozone layer in the Earth’s atmosphere filters solar radiation and limits the unwanted effects on humans. A depletion of this ozone shield would permit hazardous levels of UV solar radiation, especially in the UVB range, to bombard Earth’s surface, resulting in potentially significant effects on human health. The concern for these adverse effects intensifies if we consider that the UVB solar radiation is combined with secondary cosmic radiation (SCR) components, such as protons and muons, as well as terrestrial gamma rays. This research aims to delve into the intricate interplay between cosmic and solar radiation on earth at the cellular level, focusing on their synergistic effects on human cell biology. Through a multidisciplinary approach integrating radiobiology and physics, we aim to explore key aspects of biological responses, including cell viability, DNA damage, stress gene expression, and finally, genomic instability. To assess the impact of the combined exposure, normal i.e., non-malignant human cells (skin fibroblasts, keratinocytes, monocytes, and lymphocytes) were exposed to high-energy protons or gamma rays in combination with UVB. Cellular molecular and cytogenetic biomarkers of radiation exposure, such as DNA damage (γH2AX histone protein and dicentric chromosomes), as well as the expression pattern of various stress genes, were analyzed. In parallel, the MTS reduction and lactate dehydrogenase assays were used as indicators of cell viability, proliferation, and cytotoxicity. Results reveal remaining DNA damage for the co-exposed samples compared to samples exposed to only one type of radiation in all types of cells, accompanied by increased genomic instability and distinct stress gene expression patterns detected at 24 - 48 h post-exposure. Understanding the impact of combined radiation exposures is crucial for assessing the health risks posed to humans if the ozone layer is partially depleted, with structural and functional damages inflicted by combined cosmic and UVB exposure. |
| Kostenfreier Zugang | Open Access Gold |
| Rechteinformation | CC BY 4.0 ; Creative Commons Attribution 4.0 License |
| Förderinformationen (1) |
Förderername: European Commission (EC)
Förderer ID: 0000 0001 2242 8989 Förderer ID Typ: ISNI Titel der Förderung: 21GRD02: BIOSPHERE Förderungsnummer: 21GRD02 |
Zitierung
Gkikoudi, A., Manda, G., Beinke, C., Giesen, U., Al-Qaaod, A., Dragnea, E.-M., Dobre, M., Neagoe, I. V., Sangsuwan, T., Haghdoost, S., Vasilopoulos, S. N., Triantopoulou, S., Georgakopoulou, A., Tremi, I., Koutsoudaki, P. N., Havaki, S., Gorgoulis, V. G., Kokkoris, M., Krasniqi, F., Terzoudi, G. I., & Georgakilas, A. G. (2025). Synergistic effects of UVB and ionizing radiation on human non-malignant cells: Implications for ozone depletion and secondary cosmic radiation exposure. Biomolecules, 15(4). https:// doi.org/10.3390/biom15040536