RENEB interlaboratory comparison for biological dosimetry based on dicentric chromosome analysis and cobalt-60 exposures higher than 2.5 Gy
Autor(in); Institution
Bucher, Martin; Department of Effects and Risks of Lonising and Non-Ionising Radiation, Federal Office for Radiation Protection (BfS), Oberschleissheim, GERMANY
Endesfelder, David; Department of Effects and Risks of Lonising and Non-Ionising Radiation, Federal Office for Radiation Protection (BfS), Oberschleissheim, GERMANY
Pojtinger, Stefan; 6.2, Dosimetrie für Strahlentherapie und Röntgendiagnostik, PTB-Braunschweig
Baeyens, Ans; Radiobiology Lab, Department of Human Structure and Repair, Ghent University, Gent, BELGIUM
Barquinero, Joan-Francesc; Unitat d’Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Catalonia, SPAIN
Beinke, Christina; Bundeswehr Institute of Radiobiology, Munich, GERMANY
Bobyk, Laure; Department of Radiation Biological Effects, French Armed Forces Biomedical Research Institute, Brétigny-sur-Orge, FRANCE
Gregoire, Eric; Institut de Radioprotection et de Sureté Nucléaire (IRSN), PSE-SANTE / SERAMED / LRAcc, Fontenay-aux-Roses, FRANCE
Hristova, Rositsa; Radiobiology Department, National Centre of Radiobiology and Radiation Protection, Sofia, BULGARIA
Martinez, Juan S.; Institut de Radioprotection et de Sureté Nucléaire (IRSN), PSE-SANTE / SERAMED / LRAcc, Fontenay-aux-Roses, FRANCE
Meher, Prabodha Kumar; Center for Radiation Protection Research, Department of Molecular Biosciences, The Wenner- Gren Institute, Stockholm University, Stockholm, SWEDEN
Milanova, Marcela; Department of Radiobiology, Military Faculty of Medicine, University of Defence, Hradec Kralove, CZECH REPUBLIC
Gil, Octávia Monteiro; Centro de Ciências e Tecnologias Nucleares, Departamento de Engenharia e Ciências Nucleares, Instituto Superior Técnico (IST), Universidade de Lisboa, Lisboa, PORTUGAL
Montoro, Alegria; Service of Radiological Protection, Clinical Area of Medical Image, University and Polytechnic La Fe Hospital, Valencia, SPAIN
Moquet, Jayne; Radiation Effects Department, UK Health Security Agency, Radiation, Chemicals, Climate and Environmental Hazards Directorate, Chilton, UK
Moreno Domene, Mercedes; Laboratorio de Dosimetría Biológica. Servicio de Oncología Radioterápica, Hospital General Universitario Gregorio Marañon, Madrid, SPAIN
Prieto, María Jesús; Laboratorio de Dosimetría Biológica. Servicio de Oncología Radioterápica, Hospital General Universitario Gregorio Marañon, Madrid, SPAIN
Pujol-Canadell, Monica; Unitat d’Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, SPAIN
Sun, Mingzhu; Radiation Effects Department, UK Health Security Agency, Radiation, Chemicals, Climate and Environmental Hazards Directorate, Chilton, UK
Terzoudi, Georgia I.; Health Physics, Radiobiology and Cytogenetics Laboratory, Institute of Nuclear and Radiological Sciences and Technology, Energy and Safety, National Centre for Scientific Research ‘Demokritos’, Athens, GREECE
Tichy, Ales; Department of Radiobiology, Military Faculty of Medicine, University of Defence, Hradec Kralove, CZECH REPUBLIC
Triantopoulou, Sotiria; Health Physics, Radiobiology and Cytogenetics Laboratory, Institute of Nuclear and Radiological Sciences and Technology, Energy and Safety, National Centre for Scientific Research ‘Demokritos’, Athens, GREECE
Valente, Marco; Department of Radiation Biological Effects, French Armed Forces Biomedical Research Institute, Brétigny-sur-Orge, FRANCE
Vral, Anne; Radiobiology Lab, Department of Human Structure and Repair, Ghent University, Gent, BELGIUM
Wojcik, Andrzej; Radiobiology Department, National Centre of Radiobiology and Radiation Protection, Sofia, BULGARIA
Oestreicher, Ursula; Department of Effects and Risks of Lonising and Non-Ionising Radiation, Federal Office for Radiation Protection (BfS), Oberschleissheim, GERMANY
In previous RENEB interlaboratory comparisons based on the manual scoring of dicentric chromosomes, a tendency for systematic overestimation for doses > 2.5 Gy was found. However, these exercises included only very few doses in the high dose range, and they were heterogeneous in terms of radiation quality and evaluation mode, and comparable only to a limited extent. Here, this presumed deviation was explored by investigating three doses > 2.5 Gy. Blood samples were irradiated (2.56, 3.41 and 4.54 Gy) using a 60Co source and sent to 14 member laboratories of the RENEB network, which performed the dicentric chromosome assay (manual and/or semi-automatic scoring) and reported dose estimates. Most participants provided estimates that agreed very well with the physical reference doses and all provided dose estimates were in the correct clinical category (> 2 Gy). The previously observed tendency for a systematic bias across all laboratories was not confirmed. However, tendencies for systematic underestimation were detected for dose estimations for reference doses given in terms of absorbed dose to blood and for some participants, a laboratory-specific trend of systematic under- or overestimation was observed. The importance of regularly performed quality checks for a broad dose range became obvious to avoid misinterpretation of results.
Kostenfreier Zugang
Open Access Gold
Rechteinformation
CC BY 4.0 ; Creative Commons Attribution 4.0 License
Zitierung
Bucher, M., Endesfelder, D., Pojtinger, S., Baeyens, A., Barquinero, J.-F., Beinke, C., Bobyk, L., Gregoire, E., Hristova, R., Martinez, J. S., Meher, P. K., Milanova, M., Gil, O. M., Montoro, A., Moquet, J., Moreno Domene, M., Prieto, M. J., Pujol-Canadell, M., Sun, M., Terzoudi, G. I., Tichy, A., Triantopoulou, S., Valente, M., Vral, A., Wojcik, A., & Oestreicher, U. (2025). RENEB interlaboratory comparison for biological dosimetry based on dicentric chromosome analysis and cobalt-60 exposures higher than 2.5 Gy. Scientific Reports, 15(1), 10 S. https://doi.org/10.1038/s41598-025-89966-2
