| Zugriffsnummer | 54050 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | CCQM-P123 number and geometric property of cells adhered to a solid substrate |
| Autor(in); Institution |
Divieto, C.; INRiM, National Institute of Metrological Research,Torino, ITALY
Revel, L.; INRiM, National Institute of Metrological Research,Torino, ITALY
Pennecchi, F.; INRiM, National Institute of Metrological Research,Torino, ITALY
Pavarelli, S.; INRiM, National Institute of Metrological Research,Torino, ITALY
Zucco, M.; INRiM, National Institute of Metrological Research,Torino, ITALY
Menezes, G.; INMETRO - National Institute of Metrology Standardization and Industrial Quality, Rio de Janeiro, BRAZIL
Barrias, E.; INMETRO - National Institute of Metrology Standardization and Industrial Quality, Rio de Janeiro, BRAZIL
Leve, F.; INMETRO - National Institute of Metrology Standardization and Industrial Quality, Rio de Janeiro, BRAZIL
Lee, J.Y.; KRISS, Korea Research Institute of Standards and Science, Daejeon, REPUBLIC OF KOREA
Choi, J.-H.; KRISS, Korea Research Institute of Standards and Science, Daejeon, REPUBLIC OF KOREA
Ellison, S.L.R.; LGC Ltdt, Teddington, UNITED KINGDOM
Woolford, A.; LGC Ltdt, Teddington, UNITED KINGDOM
Lekishvili, T.; LGC Ltdt, Teddington, UNITED KINGDOM
Pang, S.; LGC Ltdt, Teddington, UNITED KINGDOM
Morley, G.; LGC Ltdt, Teddington, UNITED KINGDOM
Davies, J.; LGC Ltdt, Teddington, UNITED KINGDOM
Campbell, J.; LGC Ltdt, Teddington, UNITED KINGDOM
Braybrook, J.; LGC Ltdt, Teddington, UNITED KINGDOM
Liu, Y.; NIM, National Institute of Metrology, Beijing, CHINA
Prawettongsopen, C.; NIMT, National Institute of Metrology, Bangkok, THAILAND
Halter, M.; NIST, Gaithersburg, Maryland, USA
Elliot, J.T.; NIST, Gaithersburg, Maryland, USA
Kummrow, Andreas; 8.3, Biomedizinische Optik, PTB-Berlin
Runov, A.L.; VINIM, D.I. Mendeleyev Institute for Metrology, St. Petersburg, RUSSIA
Naschekina, Y.; VINIM, D.I. Mendeleyev Institute for Metrology, St. Petersburg, RUSSIA
Yudintseva, N.; VINIM, D.I. Mendeleyev Institute for Metrology, St. Petersburg, RUSSIA
Vonsky, M.S.; VINIM, D.I. Mendeleyev Institute for Metrology, St. Petersburg, RUSSIA
|
| Quelle/Jahr | Metrologia: 62 (2025), 1A |
| Artikelnummer | 08005 |
| ISSN | 0026-1394 (print) ; 1681-7575 (online) |
| DOI | |
| URL | |
| Verlag | Bristol: IOP Publishing |
| Freie Schlagworte | microscopy ; cell density ; confluency |
| Zusammenfassung | The CCQM-P123 study aimed to demonstrate capability of participants in quantifying 4 measurands using one reference material: number of adherent cells on a planar surface, cell density in the range 0 to 5000 cells/mm², cell confluency in the range 0 % to 100 % and average cell area. Nine Institutions participated in the study. Reference materials for three levels of cell density and cell confluency were prepared for fluorescence microscopy and circulated among participants. Each reference material was a commercial imaging dish having 400 squares and seeded with human cells, fixed and stained for nucleus and for whole cell. On each dish 4 squares were selected for the analysis as "measurement standards". The use of a calibrated ruler to define the area of measurement assured the traceability to the International System of Units (SI). Results for the 4 measurands showed an overall good agreement between the laboratories. |
Zitierung
Divieto, C., Revel, L., Pennecchi, F., Pavarelli, S., Zucco, M., Menezes, G., Barrias, E., Leve, F., Lee, J., Choi, J.-H., Ellison, S., Woolford, A., Lekishvili, T., Pang, S., Morley, G., Davies, J., Campbell, J., Braybrook, J., Liu, Y., Prawettongsopen, C., Halter, M., Elliot, J., Kummrow, A., Runov, A., Naschekina, Y., Yudintseva, N., & Vonsky, M. (2025). CCQM-P123 number and geometric property of cells adhered to a solid substrate. Metrologia, 62(1A). https://doi.org/10.1088/0026-1394/62/1A/08005