Novel analysis tool for the distance of gold dimers controlled by the DNA strand length on the DNA origami

Guckel, Jannik; Liu, Zhe; Wang, Zunhao; Lalkens, Birka; Etzkorn, Markus; Park, Daesung

doi:https://doi.org/10.1111/jmi.13371

Zugriffsnummer	53292
Dokumenttyp	Zeitschriftenartikel
Peer Review	mit Peer Review
Sprache	Englisch
Titel	Novel analysis tool for the distance of gold dimers controlled by the DNA strand length on the DNA origami
Autor(in); Institution	Guckel, Jannik; 5.2, Dimensionelle Nanometrologie, PTB-Braunschweig; Laboratory for Emerging Nanometrology (LENA), Braunschweig, GERMANY Liu, Zhe; Laboratory for Emerging Nanometrology (LENA), Braunschweig, GERMANY; Institute of Applied Physics, Technische Universität Braunschweig, Braunschweig, GERMANY Wang, Zunhao; 3.1, Allgemeine und Anorganische Chemie, PTB-Braunschweig; Laboratory for Emerging Nanometrology (LENA), Braunschweig, GERMANY Lalkens, Birka; Laboratory for Emerging Nanometrology (LENA), Braunschweig, GERMANY; Institute of Semiconductor Technology, Technische Universität Braunschweig, Braunschweig, GERMANY Etzkorn, Markus; Laboratory for Emerging Nanometrology (LENA), Braunschweig, GERMANY; Institute of Applied Physics, Technische Universität Braunschweig, Braunschweig, GERMANY Park, Daesung; 5.2, Dimensionelle Nanometrologie, PTB-Braunschweig; Laboratory for Emerging Nanometrology (LENA), Braunschweig, GERMANY
Quelle/Jahr	Journal of Microscopy: 297 (2024), 2, 215 - 226
ISSN	0022-2720 (print) ; 1365-2818 (online)
DOI	https://doi.org/10.1111/jmi.13371
Verlag	Oxford [u.a.]: Wiley-Blackwell
Freie Schlagworte	circle Hough transform ; DNA origami ; gold nanoparticles ; distance
Zusammenfassung	Metallic nanoparticle dimers have been used to enhance the excitation rate of single-quantum emitters. The interparticle distance (d) of the dimers has a crucial influence on the signal enhancement. Therefore, precise control of d is desired for optimal performance. However, statistical analysis of d has been often restricted to a small number of dimers due to the lack of reliable automatic software tools. For this reason, we developed a novel analysis tool for automatic dimer analysis. Our approach combines particle detection by circle Hough transformation (CHT) with custom classification routines optimised for distinct types of particles. We applied our tool to scanning electron microscopy (SEM) images and achieved great agreement in dimer detection, reaching an agreement of around 97% between automatic analysis and manual inspection for more than 3000 metallic nanoparticle dimers on DNA origami controlled by a combination of multiple DNA strands. Our study revealed the effects of the strand length (L) on the distribution of d. Based on geometric consideration, we expected a strong correlation between L and the standard deviation (σ) of d. We could verify this correlation by characterising four dimer designs with different L while analysing more than 1000 dimers per specimen.

Kostenfreier Zugang	Open Access Hybrid
Rechteinformation	CC BY 4.0 ; Creative Commons Attribution 4.0 License

Themenbereich der Metrologie	Metrologie in der Chemie und Stoffeigenschaften
Förderinformationen (1)	Förderername: Deutsche Forschungsgemeinschaft (DFG) Förderer ID: 0000 0001 2096 9829 Förderer ID Typ: ISNI Förderprogramm: Germany's Excellence Strategy - EXC-2123 Quantum Frontiers Förderungsnummer: 39083796
Förderinformationen (2)	Förderername: Land Niedersachsen Förderer ID: 0000 0004 0427 6733 Förderer ID Typ: ISNI Titel der Förderung: QuanTec. Förderungsnummer: 76251 (ZN 3820)
Förderinformationen (3)	Förderername: Deutsche Forschungsgemeinschaft (DFG) Förderer ID: 0000 0001 2096 9829 Förderer ID Typ: ISNI Förderungsnummer: 188/452-1 FUGG

Zitierung

Guckel, J., Liu, Z., Wang, Z., Lalkens, B., Etzkorn, M., & Park, D. (2024). Novel analysis tool for the distance of gold dimers controlled by the DNA strand length on the DNA origami. Journal of Microscopy, 297(2), 215–226. https://doi.org/10.1111/jmi.13371

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