| Zugriffsnummer | 49865 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | Towards probing conformational states of Y2 receptor using hyperpolarized 129Xe NMR |
| Autor(in); Institution |
Schmidt, P.; Institute of Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Leipzig, GERMANY
Vogel, A.; Biophysics, Medical Faculty, University of Leipzig, Leipzig, GERMANY
Schwarze, B.; Biophysics, Medical Faculty, University of Leipzig, Leipzig, GERMANY
Seufert, F.; Biophysics, Medical Faculty, University of Leipzig, Leipzig, GERMANY
Licha, K.; Institute of Chemistry and Biochemistry, Freie Universität Berlin, GERMANY
Wycisk, V.; Institute of Chemistry and Biochemistry, Freie Universität Berlin, GERMANY
Hildebrand, P.; Biophysics, Medical Faculty, University of Leipzig, Leipzig, GERMANY
|
| Quelle/Jahr | Molecules: 28 (2023), 3, 1 - 20 |
| Artikelnummer | 1424 |
| Availability | [online only] |
| ISSN | 1420-3049 (online) |
| DOI | |
| Verlag | Basel: MDPI |
| Freie Schlagworte | GPCR states ; Y2R ; NMR ; hyperpolarized xenon ; MD simulation |
| Zusammenfassung | G protein-coupled receptors can adopt many different conformational states, each of them exhibiting different restraints towards downstream signaling pathways. One promising strategy to identify and quantify this conformational landscape is to introduce a cysteine at a receptor site sensitive to different states and label this cysteine with a probe for detection. Here, the application of NMR of hyperpolarized 129Xe for the detection of the conformational states of human neuropeptide Y2 receptor is introduced. The xenon trapping cage molecule cryptophane-A attached to a cysteine in extracellular loop 2 of the receptor facilitates chemical exchange saturation transfer experiments without and in the presence of native ligand neuropeptide Y. High-quality spectra indicative of structural states of the receptor–cage conjugate were obtained. Specifically, five signals could be assigned to the conjugate in the apo form. After the addition of NPY, one additional signal and subtle modifications in the persisting signals could be detected. The correlation of the spectroscopic signals and structural states was achieved with molecular dynamics simulations, suggesting frequent contact between the xenon trapping cage and the receptor surface but a preferred interaction with the bound ligand. |
| Kostenfreier Zugang | Open Access Gold |
| Rechteinformation | CC BY 4.0 ; Creative Commons Attribution 4.0 License |
| Themenbereich der Metrologie | Metrologie in der Medizin |
Zitierung
Schmidt, P., Vogel, A., Schwarze, B., Seufert, F., Licha, K., Wycisk, V., Kilian, W., Hildebrand, P., & Mitschang, L. (2023). Towards probing conformational states of Y2 receptor using hyperpolarized 129Xe NMR. Molecules, 28(3), 1–20. https://doi.org/10.3390/molecules28031424