| Zugriffsnummer | 47607 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | All-in-one superparamagnetic and SERS-active niosomes for dual-targeted in vitro detection of breast cancer cells. |
| Autor(in); Institution |
Maurer, Viktor; Technische Universität Braunschweig, GERMANY
Zarinwall, Ajmal; Technische Universität Braunschweig, GERMANY
Wang, Zunhao; 3.1, Allgemeine und Anorganische Chemie, PTB-Braunschweig
Wundrack, Stefan; 3.1, Allgemeine und Anorganische Chemie, PTB-Braunschweig
Wundrack, Nicole; Otto von Guericke Universität Magdeburg, GERMANY
Ag Seleci, Didem; Technische Universität Braunschweig, GERMANY
Helm, Vivien; Technische Universität Braunschweig, GERMANY
Otenko, Daniil; Technische Universität Braunschweig, GERMANY
Frank, Claudia; 3.1, Allgemeine und Anorganische Chemie, PTB-Braunschweig
Schaper, Fred; Otto-von-Guericke-University Magdeburg, GERMANY
Stosch, Rainer; 3.1, Allgemeine und Anorganische Chemie, PTB-Braunschweig
Garnweitner, Georg; Technische Universität Braunschweig, Fakultät für Maschinenbau, GERMANY
|
| Quelle/Jahr | Sensors and Diagnostics: 1 (2022), 3, 469 - 484 |
| Availability | [online only] |
| ISSN | 2635-0998 (ONLINE) |
| DOI | |
| Verlag | London: Royal Society of Chemistry (RSC) |
| Freie Schlagworte | hybrid nanoparticles ; SERS ; niosomes ; diagnosis |
| Zusammenfassung | Background In vitro and in vivo biosensing through surface-enhanced Raman scattering often suffer from signal contamination diminishing both the limit of detection and quantification. However, overcoming the lack of specificity requires excessive nanoparticle concentrations, which may lead to adverse side effects if applied to patients. Results We propose encapsulation of iron oxide (FexOy) and gold (Au) nanoparticles (NPs) into the bilayer structure of transferrin-modified niosomes. This approach enables achieving greatly enhanced and contamination-free SERS-signals in vitro as well as a dual-targeting functionality towards MCF-7 breast cancer cells. An in-depth characterization of FexOyNPs- and AuNPs-loaded niosomes (AuNPs/FexOyNPs/NIO) after magnetic downstream processing reveals defined hybrid niosome structures, which show a long-term SERS-signal stability in various media such as MCF-7 cell culture medium. In vitro 2D-SERS imaging unveil a successful incorporation of a non-toxic dose of hybrid NPs into MCF-7 cells, which leads to strong and almost contamination-free SERS-signals. The measured signal-to-noise ratio of the in vitro signal exceeds the values required by DIN 32645 for the successful validation of a detection method. Conclusions The hybrid niosomes can be considered a promising and efficient agent for the establishment and commercialization of a highly sensitive detection kit for monitoring cancerous tissue. |
| Kostenfreier Zugang | Open Access Gold |
| Rechteinformation | CC BY-NC 3.0 ; Creative Commons Attribution NonCommercial 3.0 License |
| Themenbereich der Metrologie | Metrologie in der Chemie und Stoffeigenschaften |
Zitierung
Maurer, V., Zarinwall, A., Wang, Z., Wundrack, S., Wundrack, N., Ag Seleci, D., Helm, V., Otenko, D., Frank, C., Schaper, F., Stosch, R., & Garnweitner, G. (2022). All-in-one superparamagnetic and SERS-active niosomes for dual-targeted in vitro detection of breast cancer cells. Sensors and Diagnostics, 1(3), 469–484. https://doi.org/10.1039/D2SD00020B