| Zugriffsnummer | 47273 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | Investigating membrane-mediated antimicrobial peptide interactions with synchrotron radiation far-infrared spectroscopy |
| Autor(in); Institution |
Eichert, Diane M.; ELETTRA, Basovizza, Trieste, ITALY
Hoehl, Arne; 7.1, Radiometrie mit Synchrotronstrahlung, PTB-Berlin
Tiersch, Brigitte; Universität Potsdam, Potsdam, GERMANY
Ulm, Gerhard; PTB-Berlin, retired staff
Ryadnov, Maxim G.; National Physical Laboratory, Teddington, Middlesex, UK
Beckhoff, Burkhard; 7.2, Röntgenmesstechnik mit Synchrotronstrahlung, PTB-Berlin
|
| Quelle/Jahr | ChemPhysChem:(2022), 1 - 11 |
| Artikelnummer | e202100815 |
| ISSN | 1439-4235 (PRINT) ; 1439-7641 (ONLINE) |
| DOI | |
| Verlag | Weinheim: Wiley-VCH GmbH |
| Zusammenfassung | Synchrotron radiation-based Fourier transform infrared spectroscopy enables access to vibrational information from mid over far infrared to even terahertz domains. This information may prove critical for the elucidation of fundamental bio-molecular phenomena including folding-mediated innate host defence mechanisms. Antimicrobial peptides (AMPs) represent one of such phenomena. These are major effector molecules of the innate immune system, which favour attack on microbial membranes. AMPs recognise and bind to the membranes whereupon they assemble into pores or channels destabilising the membranes leading to cell death. However, specific molecular interactions responsible for antimicrobial activities have yet to be fully understood. Herein we probe such interactions by assessing molecular specific variations in the near-THz 400–40 cm−1 range for defined helical AMP templates in reconstituted phospholipid membranes. In particular, we show that a temperature-dependent spectroscopic analysis, supported by 2D correlative tools, provides direct evidence for the membrane-induced and folding-mediated activity of AMPs. The far-FTIR study offers a direct and information-rich probe of membrane-related antimicrobial interactions. |
| Kostenfreier Zugang | Open Access Hybrid |
| Rechteinformation | CC BY 4.0 ; Creative Commons Attribution 4.0 License |
| Themenbereich der Metrologie | Photometrie und Radiometrie |
| Förderinformationen (1) |
Förderername: European Commission (EC)
Förderer ID: 0000 0001 2242 8989 Förderer ID Typ: ISNI Förderprogramm: ERA-NET Plus Förderungsnummer: 217257 |
Zitierung
Hornemann, A., Eichert, D. M., Hoehl, A., Tiersch, B., Ulm, G., Ryadnov, M. G., & Beckhoff, B. (2022). Investigating membrane-mediated antimicrobial peptide interactions with synchrotron radiation far-infrared spectroscopy. ChemPhysChem, 1–11. https://doi.org/10.1002/cphc.202100815