| Zugriffsnummer | 44119 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | Total haemoglobin – a reference measuring system for improvement of standardisation |
| Autor(in); Institution |
Grote-Koska, D.; Medizinische Hochschule, Hannover, GERMANY
Klauke, R.; Medizinische Hochschule, Hannover, GERMANY
Kaiser, P.; Instand e.V., Düsseldorf, GERMANY
Kramer, U.; Instand e.V., Düsseldorf, GERMANY
Macdonald, Rainer; 8.3, Biomedizinische Optik, PTB-Berlin
Lerche, D.; Dr. Lerche KG, Berlin, GERMANY
Staaden, A.; Medizinische Hochschule, Hannover, GERMANY
Brand, K.; Medizinische Hochschule, Hannover, GERMANY
Schumann, G.; Medizinische Hochschule, Hannover, GERMANY
|
| Quelle/Jahr | Clinical Chemistry and Laboratory Medicine: 58 (2020), 8, 1314 - 1321 |
| ISSN | 1434-6621 (PRINT) ; 1437-4331 |
| DOI | |
| Verlag | Berlin: de Gruyter |
| Freie Schlagworte | haemoglobin ; metrological traceability ; reference measurement procedure ; reference measuring system ; reference method ; standardisation |
| Zusammenfassung | Background: Total haemoglobin (Hb) concentration in blood belongs to the most requested measurands, and the HiCN method (hemiglobincyanide) is accepted as a reference. Although the reaction principle is clearly characterised, measurement conditions and settings are not consistently defined, some of them influencing the results. An improvement of standardisation is the object. Methods: After method optimization, measurement results between different calibration laboratories (CL) were compared with each other and also with results of the National Metrology Institute of Germany (PTB), with target values of certified reference material, within the RELA scheme, and to )1500 results from routine laboratories. Results: Overall deviations between three CLs were ≤0.5% (n = 24 samples) in a measurement range of 20 g/L to 300 g/L. A CV of 0.4% was determined in pooled blood (1 year longterm imprecision, 99.0%–101.1% recovery of the mean). For selected measurements (n = 4 samples) the PTB participated without significant differences to three CLs, and no significant differences were observed comparing CLs to certified values of reference materials. The expanded measurement uncertainty (probability 95%) was estimated as 1.1%. Conclusions: A reference measuring system, comprising measuring instruments and other devices, including reagents and supply, to generate reference measurement values for total Hb concentration of high accuracy and low measurement uncertainty is presented. Measurement parameters are investigated and defined. The reference |
| Themenbereich der Metrologie | Metrologie in der Medizin |
Zitierung
Grote-Koska, D., Klauke, R., Kaiser, P., Kramer, U., Macdonald, R., Lerche, D., Staaden, A., Brand, K., & Schumann, G. (2020). Total haemoglobin – a reference measuring system for improvement of standardisation. Clinical Chemistry and Laboratory Medicine, 58(8), 1314–1321. https://doi.org/10.1515/cclm-2019-1177