| Zugriffsnummer | 44111 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | Initial interaction of citrate-coated iron oxide nanoparticles with the glycocalyx of THP-1 monocytes assessed by real-time magnetic particle spectroscopy and electron microscopy |
| Autor(in); Institution |
Poller, W.C.; Charité, Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Medizinische Klinik mit Schwerpunkt Kardiologie und Angiologie, Campus Mitte, Berlin, GERMANY; DZHK German Centre for Cardiovascular Research, partner site Berlin, Berlin, GERMANY
Löwa, Norbert; 8.2, Biosignale, PTB-Berlin
Schleicher, M.; Charité, Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Medizinische Klinik mit Schwerpunkt Kardiologie und Angiologie, Campus Mitte, Berlin, GERMANY; DZHK German Centre for Cardiovascular Research, partner site Berlin, Berlin, GERMANY
Münster-Wandowski, A.; Charité, Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GERMANY; Institut für Integrative Neuroanatomie, Berlin, GERMANY
Taupitz, M.; Charité, Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GERMANY; Institut für Radiologie, Campus Mitte, Berlin, GERMANY
Stangl, V.; Charité, Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Medizinische Klinik mit Schwerpunkt Kardiologie und Angiologie, Campus Mitte, Berlin, GERMANY; DZHK German Centre for Cardiovascular Research, partner site Berlin, Berlin, GERMANY
Ludwig, A.; Charité, Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Medizinische Klinik mit Schwerpunkt Kardiologie und Angiologie, Campus Mitte, Berlin, GERMANY; Charité, Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Berlin, GERMANY; Institut für Radiologie, Campus Mitte, Berlin, GERMANY
Wiekhorst, Frank; 8.2, Biosignale, PTB-Berlin
|
| Quelle/Jahr | Scientific Reports: 10 (2020), 9 S. |
| Artikelnummer | 3591 |
| Availability | [online-only] |
| ISSN | 2045-2322 |
| DOI | |
| Verlag | London: Nature Publishing Group |
| Freie Schlagworte | magnetic particle spectroscopy ; transmission electron microscopy ; glycosaminoglycans ; extracellular matrix ; cells ; very small iron-oxide nanoparticles |
| Zusammenfassung | Interaction with biological material can alter physicochemical parameters of magnetic nanoparticles and might thereby change their magnetic behavior with potentially important implications for various nanoparticle applications. Little is known about changes of the magnetic behavior that occur during the initial phase of cell binding and uptake. We investigate the magnetic behavior of very small superparamagnetic iron-oxide nanoparticles (VSOP) during initial contact with THP-1 monocytes. We combine real-time magnetic particle spectroscopy (MPS), a fast and sensitive method for specific detection of magnetic nanoparticles in biological specimen with high-pressure-freezing/freeze-substitution transmission electron microscopy (HPF/FS-TEM), enabling us to generate snapshots of the interaction of VSOP with the cellular glycocalyx. MPS reveals significant changes of the dynamic magnetic behavior within seconds after VSOP injection into monocyte suspensions that correlate with the formation of nanoparticle clusters in the glycocalyx. The combination of real-time MPS and HPF/FS-TEM provides an ideal platform to analyze magnetic behaviors of nanoparticles upon interaction with cells and tissues. |
| Kostenfreier Zugang | Open Access Gold |
| Themenbereich der Metrologie | Metrologie in der Medizin |
Zitierung
Poller, W., Löwa, N., Schleicher, M., Münster-Wandowski, A., Taupitz, M., Stangl, V., Ludwig, A., & Wiekhorst, F. (2020). Initial interaction of citrate-coated iron oxide nanoparticles with the glycocalyx of THP-1 monocytes assessed by real-time magnetic particle spectroscopy and electron microscopy. Scientific Reports, 10, 9 S. https://doi.org/10.1038/s41598-020-60162-8