| Zugriffsnummer | 43341 |
| Dokumenttyp | Konferenzartikel |
| Peer Review | unbekannt |
| Sprache | Englisch |
| Titel | Discrimination of different radiation qualities using gene expression data |
| Autor(in); Institution |
Unverricht-Yeboah, Marcus; Forschungszentrum Jülich, Department of Safety and Radiation Protection, Jülich, GERMANY
Giesen, Ulrich; 6.5, Strahlenwirkung, PTB-Braunschweig
Kriehuber, Ralf; Forschungszentrum Jülich, Department of Safety and Radiation Protection, Jülich, GERMANY
|
| Quelle/Jahr | Strahlenschutz und Medizin, Patienten - Beschäftigte - Gesellschaft: 51 (2019), 269 |
| ISSN | 1013-4506 |
| ISBN | 978-3-7406-0446-2 |
| Konferenzangaben | 51. Jahrestagung des Fachverbandes Strahlenschutz, Würzburg, 9-12, September, 2019, Deutschland |
| Freie Schlagworte | Gene expression ; ion micro beam ; Auger emitter |
| Zusammenfassung | Introduction: In order to clarify whether high-LET radiation (241Am) including Auger electrons (123IUdR) induce a generally different gene expression response when compared to low-LET radiation (137Cs) gene expression analysis covering the whole genome were carried out and analyzed. Methods: Equi-effect doses/activities, i.e. radiation doses and exposure conditions causing the same biological effect level, were determined with regard to γ-H2AX foci signal intensity (flow cytometry) 45 min after γ-irradiation (137Cs, 0.7 Gy/min: 10 Gy), α-particle exposure (241Am, 0.033 Gy/min: 1 Gy) and exposure to the Auger electron emitter 123I as 123I-iododeoxyuridine (123IUdR: 2,600 decays/cell). 123IUdR was incorporated into the DNA for 20 h. Gene expression analysis was performed employing whole human genome DNA-microarrays (Agilent) after exposure to equi-effect doses/activities. RNA for gene expression analysis was isolated 6 and 24 h post-exposure. Gene expression of selected candidate genes was validated using quantitative real-time RT-PCR. Results: At equi-effect doses/activities 155, 316 and 982 genes were exclusively regulated after exposure to 123IUdR, α-particles and γ-rays, respectively. Only four, one and one gene(s) were identified allowing a reliable and robust discrimination between γ- versus 123IUdR exposure, γ- versus α-radiation and α- versus 123IUdR exposure, respectively. The functional analysis of significantly regulated genes revealed that apoptosis and chromatin organization were most affected. Conclusions: The Auger emitter 123I induced specific gene expression patterns in Jurkat cells when compared to γ- and α-irradiation suggesting a unique cellular response after 123IUdR exposure. Gene expression profiling might be an effective tool to identify biomarkers for the discrimination of different radiation qualities and, furthermore, might help to elucidate the different biological effectiveness on the mechanistic level. |
| Themenbereich der Metrologie | Ionisierende Strahlung |
| Förderinformationen (1) |
Förderername: Bundesministerium für Bildung und Forschung (BMBF)
Förderer ID: 0000 0000 9090 0344 Förderer ID Typ: ISNI Förderungsnummer: 02NUK005A and 02NUK043A |