| Zugriffsnummer | 41979 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | Refractive index of human red blood cells between 290 nm and 1100 nm determined by optical extinction measurements |
| Autor(in); Institution |
Gienger, Jonas; 8.4, Mathematische Modellierung und Datenanalyse, PTB-Berlin
Smuda, Kathrin; 8, Medizinphysik und metrologische Informationstechnik, PTB-Berlin
Müller, Ralph; 8, Medizinphysik und metrologische Informationstechnik, PTB-Berlin
Bär, Markus; 8.4, Mathematische Modellierung und Datenanalyse, PTB-Berlin
Neukammer, Jörg; 8, Medizinphysik und metrologische Informationstechnik, PTB-Berlin
|
| Quelle/Jahr | Scientific Reports: 9 (2019), 1 - 11 |
| Artikelnummer | 4623 |
| Availability | [online only] |
| ISSN | 2045-2322 (ONLINE) |
| DOI | |
| URL | |
| Verlag | London: Springer Nature |
| Freie Schlagworte | red blood cells ; refractive index ; extinction spectroscopy ; Mie scattering |
| Zusammenfassung | The knowledge of optical properties of biological cells is essential to interpret their interaction with light and to derive morphological information and parameters associated with cell function like the oxygen transport capacity of human red blood cells (RBCs). We present a method to determine the dependence between the refractive index (RI) of human RBCs and their intracellular hemoglobin (Hb) concentration from spectral extinction measurements of a cell suspension. The procedure is based on the analysis of the corresponding ensemble averaged extinction cross section Cext(λ). Thus far two complementary approaches have been taken to derive RIs of RBCs. The first one uses homogeneous macroscopic samples prepared by hemolysis for the destruction of the RBCs’ membranes and subsequent centrifugation. A second approach is the determination of RIs of single intact cells by microscopic investigation. These techniques are limited to a few discrete wavelengths or a rather narrow wavelength range. In addition most of these techniques require additional information about the concentration dependence. In contrast, our approach yields the RI increment with Hb concentration of intact, reversibly isovolumetrically sphered, oxygenated RBCs over a wide wavelength range from 290 nm to 1100 nm from macroscopic measurements. |
| Kostenfreier Zugang | Open Access Gold |
| Themenbereich der Metrologie | Mathematik und metrologische Informationstechnik |
| Förderinformationen (1) |
Förderername: Bundesministerium für Wirtschaft und Energie (BMWi)
Förderer ID: 0000 0001 1092 3392 Förderer ID Typ: ISNI Förderprogramm: Messen, Normen, Prüfen und Qualitätssicherung (MNPQ) Titel der Förderung: Characterisation of Hb-microparticles as artificial blood substitute and development of measurement methods for control of production Förderungsnummer: MNPQ 12/14 |