| Zugriffsnummer | 40862 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | Comparative gene expression analysis after exposure to 123I-iododeoxyuridine, γ- and α-radiation - potential biomarkers for the discrimination of radiation qualities |
| Autor(in); Institution |
Unverricht-Yeboah, Marcus; Forschungszentrum Jülich, Department of Safety and Radiation Protection, Radiation Biology Unit, Jülich, GERMANY
Giesen, Ulrich; 6.5, Strahlenwirkung, PTB-Braunschweig
Kriehuber, Ralf; Forschungszentrum Jülich, Department of Safety and Radiation Protection, Radiation Biology Unit, Jülich, GERMANY
|
| Quelle/Jahr | Journal of Radiation Research: 59 (2018), 4, 411 - 429 |
| ISSN | 0449-3060 (PRINT) ; 1349-9157 (ONLINE) |
| DOI | |
| Verlag | Oxford: Oxford University Press |
| Freie Schlagworte | gene expression ; biomarker for high-LET radiation ; α-radiation ; Auger electron emitter ; 123-I-iododeoxyuridine exposure ; microarray analysis ; quantitative real-time PCR |
| Zusammenfassung | Gene expression analysis was carried out in Jurkat cells in order to identify candidate genes showing significant gene expression alterations allowing robust discrimination of the Auger emitter 123I, incorporated into the DNA as 123I-iododeoxyuridine (123IUdR), from α- and γ-radiation. The γ-H2AX foci assay was used to determine equi-effect doses or activity, and gene expression analysis was carried out at similar levels of foci induction. Comparative gene expression analysis was performed employing whole human genome DNA microarrays. Candidate genes had to show significant expression changes and no altered gene regulation or opposite regulation after exposure to the radiation quality to be compared. The gene expression of all candidate genes was validated by quantitative real-time PCR. The functional categorization of significantly deregulated genes revealed that chromatin organization and apoptosis were generally affected. After exposure to 123IUdR, α-particles and γ-rays, at equi-effect doses/activity, 155, 316 and 982 genes were exclusively regulated, respectively. Applying the stringent requirements for candidate genes, four (PPP1R14C, TNFAIP8L1, DNAJC1 and PRTFDC1), one (KLF10) and one (TNFAIP8L1) gene(s) were identified, respectively allowing reliable discrimination between γ- and 123IUdR exposure, γ- and α-radiation, and α- and 123IUdR exposure, respectively. The Auger emitter 123I induced specific gene expression patterns in Jurkat cells when compared with γ- and α-irradiation, suggesting a unique cellular response after 123IUdR exposure. Gene expression analysis might be an effective tool for identifying biomarkers for discriminating different radiation qualities and, furthermore, might help to explain the varying biological effectiveness at the mechanistic level. |
| Kostenfreier Zugang | Open Access Gold |
| Rechteinformation | CC BY-NC 4.0 ; Creative Commons Attribution NonCommercial 4.0 License |
| Förderinformationen (1) |
Förderername: Bundesministerium für Bildung und Forschung (BMBF)
Förderer ID: 0000 0000 9090 0344 Förderer ID Typ: ISNI Förderungsnummer: 02NUK005A |
| Förderinformationen (2) |
Förderername: Bundesministerium für Bildung und Forschung (BMBF)
Förderer ID: 0000 0000 9090 0344 Förderer ID Typ: ISNI Förderungsnummer: 02NUK043A |
Zitierung
Unverricht-Yeboah, M., Giesen, U., & Kriehuber, R. (2018). Comparative gene expression analysis after exposure to ¹²³I-iododeoxyuridine, γ- and α-radiation - potential biomarkers for the discrimination of radiation qualities. Journal of Radiation Research, 59(4), 411–429. https://doi.org/10.1093/jrr/rry038