| Zugriffsnummer | 40241 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | Analysis of radiation-induced chromosomal aberrations on a cell-by-cell basis after α particle microbeam irradiation: experimental data and simulations |
| Autor(in); Institution |
Testa, Antonella; Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Territorial and Production Systems Sustainability Department, Rome, ITALY
Ballarini, Francesca; University of Pavia, Physics Department, Pavia, ITALY; Italian National Institute of Nuclear Physics (INFN), Section of Pavia, ITALY
Giesen, Ulrich; 6.5, Strahlenwirkung, PTB-Braunschweig
Monteiro Gil, Octávia; Universidade de Lisboa, Centro de Ciencias e Tecnologias Nucleares, Instituto Superior Técnico, Lisbon, PORTUGAL
Carante, Mario P.; University of Pavia, Physics Department, Pavia, ITALY; Italian National Institute of Nuclear Physics (INFN), Section of Pavia, Pavia, ITALY
Tello, John; University of Pavia, Physics Department, Pavia, ITALY; Italian National Institute of Nuclear Physics (INFN), Section of Pavia, Pavia, ITALY; Universidade Estadual de Campinas, Campinas, BRAZIL
Langner, Frank; 6.5, Strahlenwirkung, PTB-Braunschweig
Rabus, Hans; 6.5, Strahlenwirkung, PTB-Braunschweig
Palma, Valentina; Territorial and Production Systems Sustainability Department, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Rome, ITALY
Pinto, Massimo; National Institute of Ionizing Radiation Metrology, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Rome, ITALY
Patrono, Clarice; Territorial and Production Systems Sustainability Department, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Rome, ITALY
|
| Quelle/Jahr | Radiation Research: 189 (2018), 6, 597 - 604 |
| ISSN | 0033-7587 |
| DOI | |
| URL | |
| Verlag | Lawrence, Kansas: Radiation Research Society |
| Freie Schlagworte | BioQuaRT ; chromosomal aberrations ; BIANCA ; ion microbeam ; radiobiology |
| Zusammenfassung | Various aspects of chromosomal aberration induction by means of ionizing radiation still need to be clarified, including correlation with the radiation track structure. Within the EMRP Joint Research Project "BioQuaRT" (Biologically weighted Quantities in Radio Therapy), an experimental and theoretical analysis was carried out on chromosomal aberrations in Chinese hamster ovary cells (CHO-K1) exposed to α-particles with final energies at the target of 5.5 MeV and 17.8 MeV (absorbed doses: ˜ 2.3 Gy and ˜ 1.9 Gy, respectively), which were generated by a microbeam available at PTB in Braunschweig (Germany). In line with the differences in LET (which was about 85 keV/μm for 5.5 MeV α particles and about 36 keV/μm for 17.8 MeV α-particles), the 5.5 MeV α-particles were more effective than the 17.8 MeV α particles, both in terms of the percentage of aberrant cells (which was 57 % vs. 33 %) and in terms of the aberration frequency. The yield of total aberrations increased by a factor of ˜ 2, although the D+R category (dicentrics plus centric rings) showed a less pronounced increase than the acentrics category. The experimental data were compared with Monte Carlo simulations based on a biophysical model called BIANCA (BIophysical ANalysis of Cell death and chromosomal Aberrations). This comparison allowed the experimental results to be interpreted in terms of critical DNA damage ("Cluster Lesions", CLs). More specifically, the higher aberration yields observed for the 5.5 MeV α particles were explained by taking into account the fact that, although the nucleus was traversed by fewer particles (nominally, 11 vs. 25), each particle was much more effective (by a factor of ˜ 3) at inducing CLs. This led to an increased yield of CLs/cell (by a factor of ˜ 1.4), consistent with the increased yield of total aberrations observed in the experiments. |
Zitierung
Testa, A., Ballarini, F., Giesen, U., Monteiro Gil, O., Carante, M. P., Tello, J., Langner, F., Rabus, H., Palma, V., Pinto, M., & Patrono, C. (2018). Analysis of radiation-induced chromosomal aberrations on a cell-by-cell basis after α particle microbeam irradiation: experimental data and simulations. Radiation Research, 189(6), 597–604. https://doi.org/10.1667/rr15005.1