| Zugriffsnummer | 37889 |
| Dokumenttyp | Konferenzartikel |
| Peer Review | unbekannt |
| Sprache | Englisch |
| Titel | Reliable determination of tissue optical properties from spatially resolved reflectance |
| Autor(in); Institution |
Gladytz, Thomas; 8.3, Biomedizinische Optik, PTB-Berlin
Hoppe, Alexander; 8.3, Biomedizinische Optik, PTB-Berlin
Cantow, K.; Charité Universitätsmedizin Berlin, GERMANY
Pohlmann, A.; Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck Center for Molecular Medicine, Berlin, GERMANY
Flemming, B.; Institut für vegetative Physiologie, Charité, Universitätsmedizin Berlin, Berlin, GERMANY
Niendorf, T.; Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck Center for Molecular Medicine, Berlin, GERMANY
Seeliger, E.; Institut für vegetative Physiologie, Charité, Universitätsmedizin Berlin, Berlin, GERMANY
Grosenick, Dirk; 8.3, Biomedizinische Optik, PTB-Berlin
|
| Quelle/Jahr | Design and Quality for Biomedical Technologies X:(2017), 100560W-1 - 100560W-9 |
| Schriftenreihe | Proceedings of SPIE: 10056 |
| Herausgeber(in) |
Raghavachari, Ramesh
Liang, Rongguang
Pfefer, T. Joshua
|
| ISSN | 0277-786X |
| ISBN | 978-1-5106-0553-4 |
| DOI | |
| Verlag | Bellingham, Wash.: SPIE |
| Konferenzangaben | Design and Quality for Biomedical Technologies X, San Francisco, Calif., 28, January - 2, February, 2017, USA |
| Zusammenfassung | Spatially resolved reflectance is a frequently used technique to derive optical properties and physiological parameters of tissue. We evaluate the accuracy of this method for source-detector distances covering a range from about 1 mm up to typically 10 mm or even 20 mm which enable us to look several millimeters deep into the scattering medium. Experimentally, we employ either a free-space light detection setup or a linear fiber probe. Measurements have been carried out on various phantoms with known optical properties derived from independently performed time-resolved measurements. Spatially resolved reflectance was analyzed by a Monte Carlo model as well as by diffusion theory for comparison. With the Monte Carlo model we also take the effects of the detection aperture into account. To evaluate the optical properties retrieved by curve fitting, we use systematic investigations of the dependency of the chi square minimization function on the optical properties. Without any calibration of the reflectance data the minimum is typically flat which results in a poor estimation of the true optical properties together with very large error bars. The separation of scattering and absorption coefficients is strongly improved when the reflectance data are calibrated. We apply our methods to in vivo investigations on renal tissue in a small animal model in which a limited number of detection fibers can be used only. Based on the estimated optical properties hemoglobin concentration and Hb oxygen saturation are quantified using the calibration approach, and uncertainties of these data are estimated. |
Zitierung
Gladytz, T., Hoppe, A., Cantow, K., Pohlmann, A., Flemming, B., Niendorf, T., Seeliger, E., & Grosenick, D. (2017). Reliable determination of tissue optical properties from spatially resolved reflectance. Design and Quality for Biomedical Technologies X, San Francisco, Calif., 28, January - 2, February, 2017, USA. https://doi.org/10.1117/12.2250552