| Zugriffsnummer | 37270 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | unbekannt |
| Sprache | Englisch |
| Titel | Non-viral magnetic engineering of endothelial cells with microRNA and plasmid-DNA - An optimized targeting approach |
| Autor(in); Institution |
Voronina, N.; Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, GERMANY
Lemcke, H.; Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, GERMANY
Wiekhorst, Frank; 8.2, Biosignale, PTB-Berlin
Kühn, J.-P.; Department of Radiology and Neuroradiology, Ernst-Moritz-Arndt-University Greifswald, Greifswald, GERMANY
Rimmbach, C.; Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, GERMANY
Steinhoff, G.; Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, GERMANY
David, R.; Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, GERMANY
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| Quelle/Jahr | Nanomedicine: Nanotechnology, Biology and Medicine: 12 (2016), 8, 2353 - 2364 |
| Availability | [online only] |
| ISSN | 1549-9642 (ONLINE) |
| DOI | |
| Verlag | Elsevier |
| Freie Schlagworte | Non-viral delivery ; microRNA ; Cell engineering ; Magnetic nanoparticles ; Magnetic targeting ; MRI |
| Zusammenfassung | Genetic modulation of angiogenesis is a powerful tool for the treatment of multiple disorders. Here, we describe a strategy to produce modified endothelial cells, which can be efficiently magnetically guided. First, we defined optimal transfection conditions with both plasmid and microRNA, using a polyethyleneimine/magnetic nanoparticle-based vector (PEI/MNP), previously designed in our group. Further, two approaches were assessed in vitro: direct vector guidance and magnetic targeting of transfected cells. Due to its higher efficiency, including simulated dynamic conditions, production of miR/PEI/MNP-modified magnetically responsive cells was selected for further detailed investigation. In particular, we have studied internalization of transfection complexes, functional capacities and intercellular communication of engineered cells and delivery of therapeutic miR. Moreover, we demonstrated that 104 miRNA/PEI/MNP-modified magnetically responsive cells loaded with 0.37 pg iron/cell are detectable with MRI. Taken together, our in vitro findings show that PEI/MNP is highly promising as a multifunctional tool for magnetically guided angiogenesis regulation. |
Zitierung
Voronina, N., Lemcke, H., Wiekhorst, F., Kühn, J.-P., Rimmbach, C., Steinhoff, G., & David, R. (2016). Non-viral magnetic engineering of endothelial cells with microRNA and plasmid-DNA - An optimized targeting approach. Nanomedicine: Nanotechnology, Biology and Medicine, 12(8), 2353–2364. http://doi.org/10.1016/j.nano.2016.06.015