| Zugriffsnummer | 37233 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | Engineering monolayer poration for rapid exfoliation of microbial membrane |
| Autor(in); Institution |
Pyne, Alice; National Physical Laboratory (NPL), Teddington, UK; National Physical Laboratory, Teddington, UK
Pfeil, Marc-Philipp; National Physical Laboratory, Teddington, UK; University of Oxford, Department of Biochemistry, Oxford, UK
Bennett, Isabel; University College, London Centre for Nanotechnology and Department of Physics and Astronomy, London, UK
Ravi, Jascindra; National Physical Laboratory (NPL), Teddington, UK
Iavicoli, Patrizia; European Comission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra, ITALY
Lamarre, Baptiste; National Physical Laboratory (NPL), Teddington, UK
Roethke, Anita; 3.1, Metrologie in der Chemie, PTB-Braunschweig
Ray, Santanu; National Physical Laboratory (NPL), Teddington, UK
Jiang, Haibo; University of Edinburgh, School of Physics and Astronomy, Edinburgh, UK
Bella, Angelo; National Physical Laboratory (NPL), Teddington, UK
Reisinger, Bernd; 3.1, Metrologie in der Chemie, PTB-Braunschweig
Yin, Daniel; National Physical Laboratory (NPL), Teddington, UK; University of Oxford, Department of Biochemistry, Oxford, UK
Little, Benjamin; IBM T.J. Watson Research Center, Yorktown Heights, USA
Muñoz-García, Juan C.; University of Oxford, Department of Biochemistry, Oxford, UK
Cerasoli, Eleonora; National Physical Laboratory (NPL), Teddington, UK
Judge, Peter J.; University of Oxford, Department of Biochemistry, Oxford, UK
Faruqui, Nilofar; National Physical Laboratory (NPL), Teddington, UK
Calzolai, Luigi; European Comission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra, ITALY
Henrion, André; 3.1, Metrologie in der Chemie, PTB-Braunschweig
Martyna, Glenn J.; IBM T.J. Watson Research Center, Yorktown Heights, USA
Grovenor, Chris R. M.; University of Oxford, Department of Materials, Oxford, USA
Crain, Jason; National Physical Laboratory (NPL), Teddington, UK; University of Edinburgh, School of Physics and Astronomy, Edinburgh, UK
Hoogenboom, Bart W.; University College, London Centre for Nanotechnology and Department of Physics and Astronomy, London, UK
Watts, Anthony; University of Oxford, Department of Biochemistry, Oxford, UK
Ryadnov, Maxim G.; National Physical Laboratory (NPL), Teddington, UK
|
| Quelle/Jahr | Chemical Science: 8 (2017), 1105 - 1115 |
| ISSN | 2041-6539 |
| DOI | |
| Verlag | Cambridge: RSC |
| Zusammenfassung | The spread of bacterial resistance to traditional a ntibiotics continues to stimulate the search for alternative ntimicrobial strategies. All forms of life, from bacteria to humans, are postulated to rely on a fundamental host defense mechanism, which exploits the formation of open pores in microbial phospholipid bilayers. Here we predict that transmembrane poration is not necessary for antimicrobial activity and reveal a distinct poration mechanism that targets the outer leaflet of phospholipid bilayers.Using a combination of molecular-scale and real-time imaging, pectroscopy and spectrometry approaches, we introduce a structural motif with a universal insertion mode in reconstituted membranes and live bacteria. We demonstrate that this motif rapidly assembles into monolayer pits that coalesce during progressive membrane exfoliation, leading to bacterial cell death within minutes. The findings offer a new physical basis for designing effective antibiotics. |
| Kostenfreier Zugang | Open Access Gold |
| Rechteinformation | CC BY-NC 3.0 ; Creative Commons Attribution NonCommercial 3.0 License |
Zitierung
Pyne, A., Pfeil, M.-P., Bennett, I., Ravi, J., Iavicoli, P., Lamarre, B., Roethke, A., Ray, S., Jiang, H., Bella, A., Reisinger, B., Yin, D., Little, B., Muñoz-García, J. C., Cerasoli, E., Judge, P. J., Faruqui, N., Calzolai, L., Henrion, A., Martyna, G. J., Grovenor, C. R. M., Crain, J., Hoogenboom, B. W., Watts, A., & Ryadnov, M. G. (2017). Engineering monolayer poration for rapid exfoliation of microbial membrane. Chemical Science, 8, 1105–1115. https://doi.org/10.1039/c6sc02925f