Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser

Ekeberg, Tomas; Svenda, Martin; Seibert, M. Marvin; Abergel, Chantal; Maia, Filipe R. N. C.; Seltzer, Virginie; DePonte, Daniel P.; Aquila, Andrew; Andreasson, Jakob; Iwan, Bianca; Jönsson, Olof; Westphal, Daniel; Odic, Dusko; Andersson, Inger; Barty, Anton; Liang, Meng; Martin, Andrew V.; Gumprecht, Lars; Fleckenstein, Holger; Bajt, Sasa; Barthelmess, Miriam; Coppola, Nicola; Claverie, Jean-Michel; Loh, N. Duane; Bostedt, Christoph; Bozek, John D.; Krzywinski, Jacek; Messerschmidt, Marc; Bogan, Michael J.; Hampton, Christina Y.; Sierra, Raymond G.; Frank, Matthias; Shoeman, Robert L.; Lomb, Lukas; Foucar, Lutz; Epp, Sascha W.; Rolles, Daniel; Rudenko, Artem; Hartmann, Robert; Hartmann, Andreas; Kimmel, Nils; Holl, Peter; Weidenspointner, Georg; Rudek, Benedikt; Erk, Benjamin; Kassemeyer, Stephan; Schlichting, Ilme; Strüder, Lothar; Ullrich, Joachim; Schmidt, Carlo; Krasniqi, Faton; Hauser, Günter; Reich, Christian; Soltau, Heike; Schorb, Sebastian; Hirsemann, Helmut; Wunderer, Cornelia; Graafsma, Heinz; Chapman, Henry; Hajdu, Janos

doi:https://doi.org/10.1038/sdata.2016.60

Zugriffsnummer	37076
Dokumenttyp	Zeitschriftenartikel
Peer Review	mit Peer Review
Sprache	Englisch
Titel	Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
Autor(in); Institution	Ekeberg, Tomas; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Uppsala, SWEDEN; Center for Free-Electron Laser Science, DESY, Hamburg, GERMANY Svenda, Martin; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Uppsala, SWEDEN Seibert, M. Marvin; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Uppsala, SWEDEN Abergel, Chantal; Information Génomique et Structurale (UMR7256) CNRS & Aix-Marseille Université, Institut de Microbiologie de la Méditerranée (FR3479), Parc Scientifique de Luminy, Marseille, FRANCE Maia, Filipe R. N. C.; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Uppsala, SWEDEN Seltzer, Virginie; Information Génomique et Structurale (UMR7256) CNRS & Aix-Marseille Université, Institut de Microbiologie de la Méditerranée (FR3479), Parc Scientifique de Luminy, Marseille, FRANCE DePonte, Daniel P.; Center for Free-Electron Laser Science, DESY, Hamburg, GERMANY; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA Aquila, Andrew; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA; European XFEL, Hamburg, GERMANY Andreasson, Jakob; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Uppsala, SWEDEN Iwan, Bianca; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Uppsala, SWEDEN; Commissariat à l’énergie atomique et aux énergies alternatives, Centre d’études de Saclay, Gif sur Yvette, FRANCE Jönsson, Olof; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Uppsala, SWEDEN; Molekyl- och kondenserade materiens fysik, Institutionen för Fysik och Astronomi, Uppsala University, Uppsala, SWEDEN Westphal, Daniel; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Uppsala, SWEDEN Odic, Dusko; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Uppsala, SWEDEN Andersson, Inger; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Uppsala, SWEDEN Barty, Anton; Center for Free-Electron Laser Science, DESY, Hamburg, GERMANY Liang, Meng; Center for Free-Electron Laser Science, DESY, Hamburg, GERMANY; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA Martin, Andrew V.; Center for Free-Electron Laser Science, DESY, Hamburg, GERMANY; The University of Melbourne, Parkville, Victoria, AUSTRALIA Gumprecht, Lars; Center for Free-Electron Laser Science, DESY, Hamburg, GERMANY Fleckenstein, Holger; Center for Free-Electron Laser Science, DESY, Hamburg, GERMANY Bajt, Sasa; Photon Science, DESY, Hamburg, GERMANY Barthelmess, Miriam; Photon Science, DESY, Hamburg, GERMANY Coppola, Nicola; Center for Free-Electron Laser Science, DESY, Hamburg, GERMANY Claverie, Jean-Michel; Information Génomique et Structurale (UMR7256) CNRS & Aix-Marseille Université, Institut de Microbiologie de la Méditerranée (FR3479), Parc Scientifique de Luminy, Marseille, FRANCE Loh, N. Duane; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA; Centre for BioImaging Sciences, National University of Singapore, Singapore, SINGAPORE Bostedt, Christoph; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA Bozek, John D.; Synchrotron SOLEIL, L’orme des Merisiers roundabout of St Aubin, Saint Aubin, FRANCE Krzywinski, Jacek; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA Messerschmidt, Marc; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA Bogan, Michael J.; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA Hampton, Christina Y.; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA Sierra, Raymond G.; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA Frank, Matthias; Lawrence Livermore National Laboratory, Livermore, California, USA Shoeman, Robert L.; Max-Planck-Institut für medizinische Forschung, Heidelberg, GERMANY Lomb, Lukas; Max-Planck-Institut für medizinische Forschung, Heidelberg, GERMANY Foucar, Lutz; Max-Planck-Institut für medizinische Forschung, Heidelberg, GERMANY; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Hamburg, GERMANY Epp, Sascha W.; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Hamburg, GERMANY; Max-Planck-Institut für Kernphysik, Heidelberg, GERMANY Rolles, Daniel; Max-Planck-Institut für medizinische Forschung, Heidelberg, GERMANY; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Hamburg, GERMANY; Department of Physics, J.R. Macdonald Laboratory, Kansas State University, Manhattan, Kansas, USA Rudenko, Artem; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Hamburg, GERMANY; Max-Planck-Institut für Kernphysik, Heidelberg, GERMANY; Department of Physics, J.R. Macdonald Laboratory, Kansas State University, Manhattan, Kansas, USA Hartmann, Robert; PNSensor GmbH, München, GERMANY Hartmann, Andreas; PNSensor GmbH, München, GERMANY Kimmel, Nils; Max-Planck-Institut Halbleiterlabor, München, GERMANY; Max-Planck-Institut für extraterrestrische Physik, Garching, GERMANY Holl, Peter; PNSensor GmbH, München, GERMANY Weidenspointner, Georg; Max-Planck-Institut Halbleiterlabor, München, GERMANY; Max-Planck-Institut für extraterrestrische Physik, Garching, GERMANY Rudek, Benedikt; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Hamburg, GERMANY; Max-Planck-Institut für Kernphysik, Heidelberg, GERMANY Erk, Benjamin; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Hamburg, GERMANY; Max-Planck-Institut für Kernphysik, Heidelberg, GERMANY Kassemeyer, Stephan; Max-Planck-Institut für medizinische Forschung, Heidelberg, GERMANY Schlichting, Ilme; Max-Planck-Institut für medizinische Forschung, Heidelberg, GERMANY; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Hamburg, GERMANY Strüder, Lothar; PNSensor GmbH, München, GERMANY; Universität Siegen, Emmy-Noether Campus, Siegen, GERMANY Ullrich, Joachim; Präsidium, P, PTB-Braunschweig; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Hamburg, GERMANY; Max-Planck-Institut für Kernphysik, Heidelberg, GERMANY Schmidt, Carlo; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Hamburg, GERMANY; Max-Planck-Institut für Kernphysik, Heidelberg, GERMANY Krasniqi, Faton; Max-Planck-Institut für medizinische Forschung, Heidelberg, GERMANY; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Hamburg, GERMANY Hauser, Günter; Max-Planck-Institut Halbleiterlabor, München, GERMANY; Max-Planck-Institut für extraterrestrische Physik, Garching, GERMANY Reich, Christian; PNSensor GmbH, München, GERMANY Soltau, Heike; PNSensor GmbH, München, GERMANY Schorb, Sebastian; Institut für Optik und Atomare Physik, Technische Universität Berlin, Berlin, GERMANY Hirsemann, Helmut; Photon Science, DESY, Hamburg, GERMANY Wunderer, Cornelia; Photon Science, DESY, Hamburg, GERMANY Graafsma, Heinz; Photon Science, DESY, Hamburg, GERMANY Chapman, Henry; Center for Free-Electron Laser Science, DESY, Hamburg, GERMANY; University of Hamburg, Hamburg, GERMANY Hajdu, Janos; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Uppsala, SWEDEN; European XFEL, Hamburg, GERMANY
Quelle/Jahr	Scientific Data: 3 (2016), 1 - 7
Artikelnummer	160060
Availability	[online only]
ISSN	2052-4463 (ONLINE)
DOI	https://doi.org/10.1038/sdata.2016.60
Verlag	London: Nature Publishing Group
Zusammenfassung	Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.

Kostenfreier Zugang	Open Access Gold
Rechteinformation	CC BY 4.0 ; Creative Commons Attribution 4.0 License

Zitierung

Ekeberg, T., Svenda, M., Seibert, M. M., Abergel, C., Maia, F. R. N. C., Seltzer, V., DePonte, D. P., Aquila, A., Andreasson, J., Iwan, B., Jönsson, O., Westphal, D., Odic, D., Andersson, I., Barty, A., Liang, M., Martin, A. V., Gumprecht, L., Fleckenstein, H., Bajt, S., Barthelmess, M., Coppola, N., Claverie, J.-M., Loh, N. D., Bostedt, C., Bozek, J. D., Krzywinski, J., Messerschmidt, M., Bogan, M. J., Hampton, C. Y., Sierra, R. G., Frank, M., Shoeman, R. L., Lomb, L., Foucar, L., Epp, S. W., Rolles, D., Rudenko, A., Hartmann, R., Hartmann, A., Kimmel, N., Holl, P., Weidenspointner, G., Rudek, B., Erk, B., Kassemeyer, S., Schlichting, I., Strüder, L., Ullrich, J., Schmidt, C., Krasniqi, F., Hauser, G., Reich, C., Soltau, H., Schorb, S., Hirsemann, H., Wunderer, C., Graafsma, H., Chapman, H., & Hajdu, J. (2016). Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser. Scientific Data, 3, 1–7. https://doi.org/10.1038/sdata.2016.60

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