| Zugriffsnummer | 36083 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | unbekannt |
| Sprache | Englisch |
| Titel | Label-free whole blood cell differentiation based on multiple frequency AC impedance and light scattering analysis in a micro flow cytometer |
| Autor(in); Institution |
Simon, Peter; 8.3, Biomedizinische Optik, PTB-Berlin
Frankowski, Marcin; 8.3, Biomedizinische Optik, PTB-Berlin
Bock, Nicole; 8.3, Biomedizinische Optik, PTB-Berlin
Neukammer, Jörg; 8.3, Biomedizinische Optik, PTB-Berlin
|
| Quelle/Jahr | Lab on a Chip: 16 (2016), 2326 - 2338 |
| ISSN | 1473-0197 (PRINT) ; 1473-0189 (ONLINE) |
| DOI | |
| Verlag | Cambridge: Royal Society of Chemistry (RSC) |
| Zusammenfassung | We developed a microfluidic sensor for label-free flow cytometric cell differentiation by combined multiple AC electrical impedance and light scattering analysis. The measured signals are correlated to cell volume,membrane capacity and optical properties of single cells. For an improved signal to noise ratio, the microfluidicsensor incorporates two electrode pairs for differential impedance detection. One-dimensionalsheath flow focusing was implemented, which allows single particle analysis at kHz count rates. Variousmonodisperse particles and differentiation of leukocytes in haemolysed samples served to benchmark themicrodevice applying combined AC impedance and side scatter analyses. In what follows, we demonstratethat AC impedance measurements at selected frequencies allow label-free discrimination of platelets,erythrocytes, monocytes, granulocytes and lymphocytes in whole blood samples involving dilution only.Immunofluorescence staining was applied to validate the results of the label-free cell analysis. Reliable differentiationand enumeration of cells in whole blood by AC impedance detection have the potential tosupport medical diagnosis for patients with haemolysis resistant erythrocytes or abnormally sensitiveleucocytes, i.e. for patients suffering from anaemia or leukaemia. |
Zitierung
Simon, P., Frankowski, M., Bock, N., & Neukammer, J. (2016). Label-free whole blood cell differentiation based on multiple frequency AC impedance and light scattering analysis in a micro flow cytometer. Lab on a Chip, 16, 2326–2338. https://doi.org/10.1039/c6lc00128a