Synthesis of acid-stabilized iron oxide nanoparticles and comparison for targeting atherosclerotic plaques: Evaluation by MRI, quantitative MPS, and TEM alternative to ambiguous Prussian blue iron staining

Scharlach, C.; Kratz, H.; Wiekhorst, Frank; Warmuth, C.; Schnorr, J.; Genter, G.; Ebert, M.; Mueller, S.; Schellenberger, E.

doi:https://doi.org/10.1016/j.nano.2015.01.002

Zugriffsnummer	35061
Dokumenttyp	Zeitschriftenartikel
Peer Review	unbekannt
Sprache	Englisch
Titel	Synthesis of acid-stabilized iron oxide nanoparticles and comparison for targeting atherosclerotic plaques: Evaluation by MRI, quantitative MPS, and TEM alternative to ambiguous Prussian blue iron staining
Autor(in); Institution	Scharlach, C.; Department of Radiology, Charité, Berlin, GERMANY Kratz, H.; Department of Radiology, Charité, Berlin, GERMANY Wiekhorst, Frank; 8.2, Biosignale, PTB-Berlin Warmuth, C.; Department of Radiology, Charité, Berlin, GERMANY Schnorr, J.; Department of Radiology, Charité, Berlin, GERMANY Genter, G.; Department of Radiology, Charité, Berlin, GERMANY Ebert, M.; Department of Radiology, Charité, Berlin, GERMANY Mueller, S.; Department of Experimental Neurology, Center for Stroke Research Berlin (CSB), Charité, Berlin, GERMANY Schellenberger, E.; Department of Radiology, Charité, Berlin, GERMANY
Quelle/Jahr	Nanomedicine: Nanotechnology, Biology and Medicine: 11 (2015), 5, 1085 - 1095
Availability	[online only]
ISSN	1549-9642
DOI	https://doi.org/10.1016/j.nano.2015.01.002
Verlag	Amsterdam: Elsevier
Freie Schlagworte	Atherosclerotic plaques ; Iron oxide particles ; Magnetic resonance imaging ; Magnetic particle spectroscopy ; Ferumoxytol
Zusammenfassung	To further optimize citrate-stabilized VSOPs (very small iron oxide particles, developed for MR angiography) for identification of atherosclerotic plaques, we modified their surface during synthesis using eight other acids for electrostatic stabilization. This approach preserves effective production for clinical application. Five particles were suitable to be investigated in targeting plaques of apoE^−/− mice. Accumulation was evaluated by ex vivo MRI, TEM, and quantitatively by magnetic particle spectroscopy (MPS). Citric- (VSOP), etidronic-, tartaric-, and malic-acid-coated particles accumulated in atherosclerotic plaques with highest accumulation for VSOP (0.2‰ of injected dose). Targets were phagolysosomes of macrophages and of altered endothelial cells. In vivo MRI with VSOP allowed for definite plaque identification. Prussian blue staining revealed abundant endogenous iron in plaques, indistinguishable from particle iron. In apoE^−/− mice, VSOPs are still the best anionic iron oxide particles for imaging atherosclerotic plaques. MPS allows for quantification of superparamagnetic nanoparticles in such small specimens.

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Scharlach, C., Kratz, H., Wiekhorst, F., Warmuth, C., Schnorr, J., Genter, G., Ebert, M., Mueller, S., & Schellenberger, E. (2015). Synthesis of acid-stabilized iron oxide nanoparticles and comparison for targeting atherosclerotic plaques: Evaluation by MRI, quantitative MPS, and TEM alternative to ambiguous Prussian blue iron staining. Nanomedicine: Nanotechnology, Biology and Medicine, 11(5), 1085–1095. https://doi.org/10.1016/j.nano.2015.01.002

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