| Zugriffsnummer | 33936 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | unbekannt |
| Sprache | Englisch |
| Titel | Qualifying label components for effective biosensing using advanced high-throughput SEIRA methodology |
| Autor(in); Institution |
Hornemann, Andrea; 7.2, Kryophysik und Spektrometrie, PTB-Berlin
Eichert, Diane; Elettra-Sincrotrone, Trieste, ITALY
Fleming, Sabine; BAM Federal Institute for Materials Research and Testing, Berlin, GERMANY
Ulm, Gerhard; 7, Temperatur und Synchrotronstrahlung, PTB-Berlin
Beckhoff, Burkhard; 7.2, Kryophysik und Spektrometrie, PTB-Berlin
|
| Quelle/Jahr | Physical Chemistry, Chemical Physics:(2015), 17, 9471 - 9479 |
| ISSN | 1463-9076 |
| DOI | |
| URL | |
| Verlag | Cambridge: Royal Soc. of Chemistry |
| Klassifikationscode | OCIS: 120.0120 ; OCIS: 240.0240 ; OCIS: 300.0300 ; OCIS: 310.6860 ; OCIS: 180.4243 |
| Zusammenfassung | The need for technological progress in bio-diagnostic assays of high complexity requires both fundamental research and constructing efforts on nano-scaled assay recognition elements that can provide unique selectivity and design-enhanced sensitivity features. Nanoparticle induced sensitivity enhancement and its application related to multiplexed capability Surface-Enhanced InfraRed Absorption (SEIRA) assay formats are well suitable for these purposes. The potential of diverse fluorophore–antibody conjugates, being chemisorbed onto low-cost gold nanoparticulate SEIRA substrates, has been explored with respect to their spectral discriminability. These novel biolabels deliver molecular SEIRA fingerprints that have been successfully analyzed by both uni- and multivariate analyzing tools, to discriminate their multiplexing capabilities. We show that this robust spectral encoding via SEIRA fingerprints opens up new opportunities for a fast, reliable and multiplexed high-end screening in biodiagnostics. |
