| Zugriffsnummer | 33857 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | unbekannt |
| Sprache | Englisch ; Deutsch |
| Titel | Survey results on nucleic acid tests of infectious diseases:present status and need for rapid and near-patient diagnostics |
| Übersetzungstitel | Umfrage zu Nukleinsäureamplifikationstests bei Infektionserkrankungen: Stand und Bedarf für eine schnelle und patientennahe Diagnostik |
| Autor(in); Institution |
Neukammer, Jörg; 8.3, Biomedizinische Optik, PTB-Berlin
Hussels, Martin; 8.3, Biomedizinische Optik, PTB-Berlin
Kummrow, Andreas; 8.3, Biomedizinische Optik, PTB-Berlin
Devonshire, A.; LGC Limited, Teddington, Middlesex, UK
Foy, C.; LGC Limited, Teddington, Middlesex, UK
Huggett, J.; LGC Limited, Teddington, Middlesex, UK
Parkes, Helen; LGC Limited, Teddington, Middlesex, UK
Zel, J.; National Institute of Biology, Ljubljana, SLOVENIA
Milavec, M.; National Institute of Biology, Ljubljana, SLOVENIA
Schimmel, H.; European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Geel, BELGIUM
Unger, W.; Bundesanstalt für Materialforschung und -prüfung (BAM), Berlin, GERMANY
Akgöz, M.; Türkiye Bilimsel ve Teknolojik Arastirma Kurumu - Ulusal Metroloji Enstitüsü TUBITAK UME, Gebze/Kocaeli, TURKEY
|
| Quelle/Jahr | GMS Zeitschrift zur Förderung der Qualitätssicherung in medizinischen Laboratorien: 6 (2015), 25 S. |
| ISSN | 1869-4241 |
| DOI | |
| Persistent Identifier | |
| Verlag | Düsseldorf: GMS |
| Freie Schlagworte | nucleic acid tests ; infectious diseases ; virus detection ; bacteria detection ; isothermal nucleic acid amplification ; status report ; questionnaire ; NAT ; PCR |
| Zusammenfassung | This survey discusses current and emerging isothermal and rapid poly-merase chain reaction (PCR) based nucleic acid amplification methods for near-patient diagnostics. To assess the clinical need of rapid diagnostics for infectious diseases based on nucleic acid tests (NATs) we performed and analysed a questionnaire among laboratories participating in corresponding INSTAND ring trials for external quality assurance. The questions concerning new amplification technologies like isothermal nucleic acid amplification, potentially suited to significantly decrease turnaround times, were complemented by questions to evaluate the present Status of NATs. Besides end-users, companies were also addressed by sending out a manufacturer specific questionnaire. Analysis of the answers from 48 laboratories in 14 European countries revealed that a much shorter turnaround time is requested for selected pathogens compared to about 2 h or longer when applying temperature cycling amplification, i.e. PCR. In this context, most frequently mentioned were methicillin-resistant Staphylococcus aureus (MRSA), norovirus, influenza A and B viruses, cytomegalovirus (CMV) as well as Hepatitis B virus (HBV) and hepatitis C virus (HCV). At present, 8% of the laboratories having participated in this survey apply isothermal amplification of nucleic acids to identify infectious pathogens. |
| Kostenfreier Zugang | Freier Zugang |
| Rechteinformation | CC BY 4.0 ; Creative Commons Attribution 4.0 License |
Zitierung
Neukammer, J., Hussels, M., Kummrow, A., Devonshire, A., Foy, C., Huggett, J., Parkes, H., Zel, J., Milavec, M., Schimmel, H., Unger, W., & Akgöz, M. (2015). Survey results on nucleic acid tests of infectious diseases:present status and need for rapid and near-patient diagnostics. GMS Zeitschrift zur Förderung der Qualitätssicherung in medizinischen Laboratorien, 6, 25 S. https://doi.org/10.3205/lab000016