| Zugriffsnummer | 31442 |
| Dokumenttyp | Konferenzartikel |
| Sprache | Englisch |
| Titel | Multienzyme biosensors - coupled enzyme reactions and enzyme activation |
| Autor(in); Institution |
Scheller, Frieder; Max-Delbrück-Center of Molecular Medicine, Berlin, GERMANY
Wollenberger, Ulla; Max-Delbrück-Center of Molecular Medicine, Berlin, GERMANY
Schubert, Florian; 10.12, Messtechnik in der Laboratoriumsmedizin, PTB-Berlin
Pfeiffer, Dorothea; University of Newcastel, Newcastel upon Tyne, UK
Makower, Alexander; University of Newcastel, Newcastel upon Tyne, UK
McNeil, C.; University of Newcastel, Newcastel upon Tyne, UK
|
| Quelle/Jahr | Uses of immobilized biological compounds:(1993), 171 - 179 |
| Schriftenreihe | NATO ASI series : Series E, Applied sciences: 252 |
| Herausgeber(in) |
Guilbault, George G.; Department of Chemistry, University of New Orleans, La., USA
Mascini, Marco; Institute of Analytical Chemistry, University of Florence, ITALY
|
| ISBN | 0-7923-2529-X |
| DOI | |
| Verlag | Dordrecht [u.a.]: Kluwer |
| Konferenzangaben | NATO Advanced Research Workshop on Uses of Immobilized Biological Compounds for Detection, Medical, Food and Environmental Analysis, Brixen, 09-14, May, 1993, Italy |
| Zusammenfassung | Traditionally enzymes are used as recognition elements to measure substrate molecules by catalyzing the turnover of these species to detectable products. The signal generation at biosensors using immobilized biomolecules is based on the superposition of the following processes: (i) the mass transport of the analyte, (ii) biochemical recognition of the substance to be measured, and (iii) generation of the electrical output at the transducer interface. Optimal sensitivity for substrate measurement is reached at stoichometric analyte conversion within a highly permeable enzyme layer. On the other hand, substances which effect the enzyme activity get accessible to the determination by enzyme sensors provided that the overall signal is governed by the enzyme reaction. Despite the low sensitivity towards the substrate these kinetically controlled sensors allow the indication of submicromolar concentrations of inhibitors and activators. |
Zitierung
Scheller, F., Wollenberger, U., Schubert, F., Pfeiffer, D., Makower, A., & McNeil, C. (1993). Multienzyme biosensors - coupled enzyme reactions and enzyme activation. NATO Advanced Research Workshop on Uses of Immobilized Biological Compounds for Detection, Medical, Food and Environmental Analysis, Brixen, 09-14, May, 1993, Italy. https://doi.org/10.1007/978-94-011-1932-0_17