| Zugriffsnummer | 31339 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | unbekannt |
| Sprache | Englisch |
| Titel | Enhancing biosensor performance using multienzyme systems |
| Autor(in); Institution |
Wollenberger, Ulla; Fraunhofer Institute for Silicon Technology, Berlin, GERMANY
Schubert, Florian; 10.12, Messtechnik in der Laboratoriumsmedizin, PTB-Berlin
Pfeiffer, Dorothea; Max-Delbrück-Center for Molecular Medicine, Berlin, GERMANY
Scheller, Frieder W.; Max-Delbrück-Center for Molecular Medicine, Berlin, GERMANY
|
| Quelle/Jahr | Trends in Biotechnology: 11 (1993), 6, 255 - 262 |
| ISSN | 0167-7799 (PRINT) ; 1879-3096 (ONLINE) |
| DOI | |
| Verlag | Cambridge: Elsevier |
| Zusammenfassung | Enhancing the performance of biosensors, in terms of increasing the range of analytes that may be detected, and the sensitivity and specificity of the detection event, would improve the prospects for commercializing this technology. Coupling the catalytic activities of several enzymes is one approach being used to address these issues. Sequences of enzymes, where ligand binding triggers the activation of enzymes, or where biocatalytic pre-concentration of intermediates permits augmentation of the signal, may be used. In addition, enzymatic recycling of the analyte can be used to increase the sensitivity by several orders of magnitude. |
