| Zugriffsnummer | 31300 |
| Dokumenttyp | Zeitschriftenartikel |
| Sprache | Englisch |
| Titel | A fiber-optic enzyme sensor for the determination of adenosine diphosphate using internal analyte recycling |
| Autor(in); Institution |
Schubert, Florian; 10.12, Messtechnik in der Laboratoriumsmedizin, PTB-Berlin
|
| Quelle/Jahr | Sensors and actuators B: 11 (1993), 1-3, 531 - 535 |
| ISSN | 0925-4005 (PRINT) ; 1873-3077 (ONLINE) |
| DOI | |
| Verlag | Amsterdam [u.a.]: Elsevier |
| Zusammenfassung | An optical mutlienzyme biosensor for the sensitive determination of adenosine diphosphate (ADP) is presented. Hexokinase, pyruvate kinase, and glucose-6-phosphate dehydrogenase are coimmobilized in a polyurethane membrane and fixed to a commercial fiber-optic probe. ADP is continuously phosphorylated/dephosphorylated in a cycle catayzed by the kinases. The glucose-6-phospahte formed in the hexokinase reaction is oxidized by the dehydrogenase, leading to the formation of NADH, the fluorescence of which is detected by the optical transducer and used as the measuring signal. Comparison of the responses of the sensor to adenosine triphosphate (ATP) and ADP shows and amplification factor of 90 for ADP measurement. The detection limit is thus lowered to 0.1 μM, the useful measuring range extending up to 20 μM. The response of the sensor to other compounds involved in the reaction system has also been investigated. |
