| Zugriffsnummer | 30673 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | Towards traceable size determination of extracellular vesicles |
| Autor(in); Institution |
Varga, Zoltan; Department of Biological Nanochemistry, Institute of Molecular Pharmacology, Research Centre for Natural sciences, Hungarian Academy of Sciences, Budapest, HUNGARY
Yuana, Yuana; Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, THE NETHERLANDS
Grootemaat, Anita E.; Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, THE NETHERLANDS
Pol, Edwin van der; Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, THE NETHERLANDS; Department of Biomedical Engineering and Physics, Academic Medical Centre of the University of Amsterdam, Amsterdam, THE NETHERLANDS
Gollwitzer, Christian; 7.1, Radiometrie mit Synchrotronstrahlung, PTB-Berlin
Krumrey, Michael; 7.1, Radiometrie mit Synchrotronstrahlung, PTB-Berlin
Nieuwland, Rienk; Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, THE NETHERLANDS
|
| Quelle/Jahr | Journal of Extracellular Vesicles: 3 (2014), 1 - 10 |
| ISSN | 2001-3078 |
| DOI | |
| Verlag | [S.l.]: Co-Action Publ. |
| Freie Schlagworte | exosome ; microvesicle ; erythrocyte ; extracellular vesicles ; freeze-fracture transmission electron microscopy ; nanoparticle tracking analysis ; resistive pulse sensing ; small-angle X-ray scattering ; dynamic light scattering ; size exclusion chromatography |
| Zusammenfassung | Background: Extracellular vesicles (EVs) have clinical importance due to their roles in a wide range of biological processes. The detection and characterization of EVs are challenging because of their small size, low refractive index, and heterogeneity. Methods: In this manuscript, the size distribution of an erythrocyte-derived EV sample is determined using state-of-the-art techniques such as nanoparticle tracking analysis, resistive pulse sensing, and electron microscopy, and novel techniques in the field, such as small-angle X-ray scattering (SAXS) and size exclusion chromatography coupled with dynamic light scattering detection. Results: The mode values of the size distributions of the studied erythrocyte EVs reported by the different methods show only small deviations around 130 nm, but there are differences in the widths of the size distributions. Conclusion: SAXS is a promising technique with respect to traceability, as this technique was already applied for traceable size determination of solid nanoparticles in suspension. To reach the traceable measurement of EVs, monodisperse and highly concentrated samples are required. |
| Kostenfreier Zugang | Open Access Gold |
| Rechteinformation | CC BY-NC 3.0 ; Creative Commons Attribution NonCommercial 3.0 License |