| Zugriffsnummer | 29050 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | mit Peer Review |
| Sprache | Englisch |
| Titel | Microflow cytometers with integrated hydrodynamic focusing |
| Autor(in); Institution |
Frankowski, Marcin; 8.3, Biomedizinische Optik, PTB-Berlin
Theisen, J.; Technische Universität Berlin, Berlin, GERMANY
Kummrow, Andreas; 8.3, Biomedizinische Optik, PTB-Berlin
Simon, Peter; 8.3, Biomedizinische Optik, PTB-Berlin
Ragusch, Hülya; 8.3, Biomedizinische Optik, PTB-Berlin
Bock, Nicole; 8.3, Biomedizinische Optik, PTB-Berlin
Schmidt, M.; Technische Universität Berlin, Berlin, GERMANY
Neukammer, Jörg; 8.3, Biomedizinische Optik, PTB-Berlin
|
| Quelle/Jahr | Sensors: 13 (2013), 4, 4674 - 4693 |
| Availability | [online only] |
| ISSN | 1424-8220 |
| DOI | |
| Verlag | Basel: MDPI |
| Freie Schlagworte | microfluidics ; flow cytometry ; lab-on-a-chip ; single cell analysis ; blood cell |
| Zusammenfassung | This study demonstrates the suitability of microfluidic structures for high throughput blood cell analysis. The microfluidic chips exploit fully integrated hydrodynamic focusing based on two different concepts: Two-stage cascade focusing and spin focusing (vortex) principle. The sample - A suspension of micro particles or blood cells - is injected into a sheath fluid streaming at a substantially higher flow rate, which assures positioning of the particles in the center of the flow channel. Particle velocities of a few m/s are achieved as required for high throughput blood cell analysis. The stability of hydrodynamic particle positioning was evaluated by measuring the pulse heights distributions of fluorescence signals from calibration beads. Quantitative assessment based on coefficient of variation for the fluorescence intensity distributions resulted in a value of about 3% determined for the micro-device exploiting cascade hydrodynamic focusing. For the spin focusing approach similar values were achieved for sample flow rates being 1.5 times lower. Our results indicate that the performances of both variants of hydrodynamic focusing suit for blood cell differentiation and counting. The potential of the micro flow cytometer is demonstrated by detecting immunologically labeled CD3 positive and CD4 positive T-lymphocytes in blood. |
| Kostenfreier Zugang | Open Access Gold |
| Rechteinformation | CC BY 3.0 ; Creative Commons Attribution 3.0 License |