| Zugriffsnummer | 28844 |
| Dokumenttyp | Zeitschriftenartikel |
| Sprache | Englisch |
| Titel | Improving species-specific IDMS: the advantages of triple IDMS. |
| Autor(in); Institution |
Frank, Claudia; 3.1, Metrologie in der Chemie, PTB-Braunschweig
Rienitz, Olaf; 3.1, Metrologie in der Chemie, PTB-Braunschweig
Swart, Claudia; 3.1, Metrologie in der Chemie, PTB-Braunschweig
Schiel, Detlef; 3.1, Metrologie in der Chemie, PTB-Braunschweig
|
| Quelle/Jahr | Analytical and Bioanalytical Chemistry: 405 (2013), 6, 1913 - 1919 |
| ISSN | 1618-2642 (PRINT) ; 1618-2650 (ONLINE) |
| DOI | |
| Verlag | Berlin [u.a.]: Springer |
| Freie Schlagworte | transferrin ; species specific IDMS ; triple IDMS ; human serum |
| Zusammenfassung | Triple isotope dilution mass spectrometry (triple IDMS) has been applied the first time on protein quantification, especially on transferrin. Transferrin as acute phase protein is a marker for several inflammation processes in the human body. Therefore, in Germany the accurate and precise measurement of this important analyte is required. In this work, a new approach of triple IDMS is described and compared to double IDMS. Also complete uncertainty budgets for both methods were set up to demonstrate the ability of this method to be used as reference procedure. The relative expanded uncertainty (k = 2) for triple IDMS (3.6 %) is smaller than the one for double IDMS (4.0 %). The content of transferrin found in the human serum reference material ERM-DA470k/IFCC ((2.41 ± 0.08) g/kg) with both methods was in good agreement with each other and with the certificate. For triple IDMS (2.426 ± 0.086) g/kg and for double IDMS (2.317 ± 0.092) g/kg), transferrin was determined. Although triple IDMS is a little more time consuming compared to double IDMS, there is the advantage that the isotopic composition of the spike material does not have to be determined. This is very useful especially in case of a marginal isotopic enrichment in the spike or problems with the accurate measurement of the spike isotope ratio. Figure using triple instead of double species-specific IDMS helps to reduce the uncertainty and improves the reliablity of the results, especially in cases where an accurate determination of the spike isotope ratio is difficult or impossible, because the spike ratio cancels from the equation. |
