| Zugriffsnummer | 24367 |
| Dokumenttyp | Zeitschriftenartikel |
| Sprache | Englisch |
| Titel | The RBE of 3.4 MeV α-particles and 0.565 MeV neutrons relative to 60Co γ-rays for neoplastic transformation of human hybrid cells and the impact of culture conditions |
| Autor(in); Institution |
Frankenberg-Schwager, M.; Universität Göttingen, Abteilung Nuklearmedizin im Zentrum Radiologie, Göttingen, GERMANY
Spieren, S; Universität Göttingen, Abteilung Klinische Strahlenbiologie und Klinische Strahlenphysik im Zentrum Radiologie, Göttingen, GERMANY
Pralle, E.; Universität Göttingen, Abteilung Klinische Strahlenbiologie und Klinische Strahlenphysik im Zentrum Radiologie, Göttingen, GERMANY
Giesen, Ulrich; 6.4, Ionenbeschleuniger und Referenzstrahlungsfelder, PTB-Braunschweig
Brede, Hein Jürgen; PTB-Braunschweig, retired staff
Thiemig, Michael; 6.4, Ionenbeschleuniger und Referenzstrahlungsfelder, PTB-Braunschweig
Frankenberg, Dieter; PTB-Braunschweig, retired staff
|
| Quelle/Jahr | Radiation Protection Dosimetry: 138 (2010), 1, 29 - 39 |
| ISSN | 0144-8420 |
| DOI | |
| Verlag | Oxford: Oxford University Press |
| Freie Schlagworte | Neoplastic Transformation ; CGL1 cells ; relative biological effectiveness (RBE) ; neutron radiation ; CO-60 |
| Zusammenfassung | The neoplastic transformation of human hybrid CGL1 cells is affected by perturbations from external influences such as serum batch and concentration, the number of medium changes during the 21-day expression period and cell seeding density. Nevertheless, for doses up to 1.5 Gy, published transformation frequencies for low linear energy transfer (LET) radiations (γ-rays, MeV electrons or photons) are in good agreement, whereas for higher doses larger variations are reported. The 60Co γ-ray data here for doses up to 1.5 Gy, using a low-yield serum batch and only one medium change, are in agreement with published frequencies of neoplastic transformation of human hybrid cells. For 3.4 MeV α-particles (LET = 124 keV/μm) and 0.565 MeV monoenergetic neutrons relative to low doses of 60Co γ-rays, a maximum relative biological effectiveness (RBEM) of 2.8 ± 0.2 and 1.5 ± 0.2, respectively, was calculated. Surprisingly, at higher doses of 60Co γ-rays lower frequencies of neoplastic transformation were observed. This non-monotonic dose relationship for neoplastic transformation by 60Co γ-rays is likely due to the lack of a G2/M arrest observed at low doses resulting in higher transformation frequencies per dose, whereas the lower frequencies per dose observed for higher doses are likely related to the induction of a G2/M arrest. |
Zitierung
Frankenberg-Schwager, M., Spieren, S., Pralle, E., Giesen, U., Brede, H. J., Thiemig, M., & Frankenberg, D. (2010). The RBE of 3.4 MeV α-particles and 0.565 MeV neutrons relative to 60Co γ-rays for neoplastic transformation of human hybrid cells and the impact of culture conditions. Radiation Protection Dosimetry, 138(1), 29–39. https://doi.org/10.1093/rpd/ncp201