| Zugriffsnummer | 22902 |
| Dokumenttyp | Zeitschriftenartikel |
| Peer Review | unbekannt |
| Sprache | Englisch |
| Titel | Combined targeting of lentiviral vectors and positioning of transduced cells by magnetic nanoparticles |
| Autor(in); Institution |
Hofmann, Andreas; University of Bonn, Institute for Pharmacology and Toxicology, Bonn, GERMANY
Wenzel, Daniela; University of Bonn, Institute of Physiology I, Bonn, GERMANY
Becher, Ulrich M.; University of Bonn, Department of Internal Medicine II, Bonn, GERMANY
Freitag, Daniel F.; University of Bonn, Institute of Physiology I, Bonn, GERMANY
Klein, Alexandra M.; University of Bonn, Institute of Physiology I, Bonn, GERMANY
Eberbeck, Dietmar; 8.2, Biosignale, PTB-Berlin
Schulte, Maike; University of Bonn, Institute for Pharmacology and Toxicology, Bonn, GERMANY
Zimmermann, Katrin; University of Bonn, Institute for Pharmacology and Toxicology, Bonn, GERMANY
Bergemann, Christian; Chemicell GmbH, Berlin, GERMANY
Gleich, Bernhard; TU Munich, Zentralinstitut für Medizintechnik (IMETUM), München, GERMANY
Roell, Wilhelm; University of Bonn, Cardiac Surgery, Bonn, GERMANY
Weyh, Thomas; TU Munich, Zentralinstitut für Medizintechnik (IMETUM), München, GERMANY
Trahms, Lutz; 8.2, Biosignale, PTB-Berlin
Nickenig, Georg; University of Bonn, Department of Internal Medicine II, Bonn, GERMANY
Fleischmann, Bernd K.; University of Bonn, Pharma Center Bonn, Bonn, GERMANY
Pfeifer, Alexander; University of Bonn, Institute for Pharmacology and Toxicology, Bonn, GERMANY
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| Quelle/Jahr | Proceedings of the National Academy of Sciences of the United States of America: 106 (2009), 1, 44 - 49 |
| ISSN | 0027-8424 (PRINT) ; 1091-6490 (ONLINE) |
| DOI | |
| URL | |
| Verlag | Washington, DC: The National Academy of Sciences of the USA |
| Freie Schlagworte | cell positioning ; gene transfer ; vector targeting |
| Zusammenfassung | Targeting of viral vectors is a major challenge for in vivo gene delivery, especially after intravascular application. In addition, targeting of the endothelium itself would be of importance for gene-based therapies of vascular disease. Here, we used magnetic nanoparticles (MNPs) to combine cell transduction and positioning in the vascular system under clinically relevant, nonpermissive conditions, including hydrodynamic forces and hypothermia. The use of MNPs enhanced transduction efficiency of endothelial cells and enabled direct endothelial targeting of lentiviral vectors (LVs) by magnetic force, even in perfused vessels. In addition, application of external magnetic fields to mice significantly changed LV/MNP biodistribution in vivo. LV/MNP-transduced cells exhibited superparamagnetic behavior as measured by magnetorelaxometry, and they were efficiently retained by magnetic fields. The magnetic interactions were strong enough to position MNP-containing endothelial cells at the intima of vessels under physiological flow conditions. Importantly, magnetic positioning of MNPlabeled cells was also achieved in vivo in an injury model of the mouse carotid artery. Intravascular gene targeting can be combined with positioning of the transduced cells via nanomagnetic particles, thereby combining gene- and cell-based therapies. |
Zitierung
Hofmann, A., Wenzel, D., Becher, U. M., Freitag, D. F., Klein, A. M., Eberbeck, D., Schulte, M., Zimmermann, K., Bergemann, C., Gleich, B., Roell, W., Weyh, T., Trahms, L., Nickenig, G., Fleischmann, B. K., & Pfeifer, A. (2009). Combined targeting of lentiviral vectors and positioning of transduced cells by magnetic nanoparticles. Proceedings of the National Academy of Sciences of the United States of America, 106(1), 44–49. https://doi.org/10.1073/pnas.0803746106