| Zugriffsnummer | 20770 |
| Dokumenttyp | Zeitschriftenartikel |
| Sprache | Englisch |
| Titel | Optical molecular imaging of lymph nodes using a targeted vascular contrast agent |
| Autor(in); Institution |
Licha, K.; Schering AG, Berlin, GERMANY
Debus, N.; Schering AG, Berlin, GERMANY
Emig-Vollmer, S.; Schering AG, Berlin, GERMANY
Hofmann, B.; Schering AG, Berlin, GERMANY
Hasbach, M.; Schering AG, Berlin, GERMANY
Stibenz, D.; Schering AG, Berlin, GERMANY
Sydow, S.; Schering AG, Berlin, GERMANY
Schirner, M.; Schering AG, Berlin, GERMANY
Ebert, Bernd; 8.3, Biomedizinische Optik, PTB-Berlin
Petzelt, Diethard; 8.3, Biomedizinische Optik, PTB-Berlin
Bührer, C.; Charité – Universitätsmedizin Berlin, GERMANY
Semmler, Wolfhard; Deutsches Krebsforschungszentrum, Heidelberg, GERMANY
Tauber, R.; Charité – Universitätsmedizin Berlin, GERMANY
|
| Quelle/Jahr | Journal of Biomedical Optics: 10 (2005), 4, 041205-1 - 041205-6 |
| ISSN | 1083-3668 |
| DOI | |
| Verlag | Bellingham, Wash.: SPIE |
| Klassifikationscode | PACS: 87.63.Lk ; PACS: 42.62.Be ; PACS: 87.14.Ee ; PACS: 87.15.Mi |
| Zusammenfassung | We develop a highly specific antibody-dye conjugate for optical imaging of peripheral lymph nodes. The contrast agent consists of the monoclonal antibody recognizing endothelial ligands for the lymphocyte homing receptor L-selectin, MECA-79, and a near-infrared (near-IR) fluorescent indotricarbocyanine dye. The targeting and biodistribution behavior of MECA-79 is studied after radio-iodination and intravenous injection into mice demonstrating specific uptake in lymph nodes and accumulation in high endothelial venules (HEV). After conjugation of MECA-79 with indotricarbocyanine dye, the fluorescence imaging properties of the MECA-79 dye conjugate are examined by intravenous injection in nude mice and laser-induced fluorescence whole-body imaging in vivo. The MECA-79 antibody-dye conjugate accumulates in peripheral lymph nodes, whereas an isotype antibody-dye conjugate does not. Specific lymph node near-IR fluorescent signals become detectable within minutes after injection, and stable imaging persists for more than 24 h. The results demonstrate that vascular targeting of endothelial expression of glyocproteins is feasible to visualize the accumulation of near-IR fluorescent MECA-79 in lymph nodes, making this technology potentially useful to characterize processes of inflammation. |
Zitierung
Licha, K., Debus, N., Emig-Vollmer, S., Hofmann, B., Hasbach, M., Stibenz, D., Sydow, S., Schirner, M., Ebert, B., Petzelt, D., Bührer, C., Semmler, W., & Tauber, R. (2005). Optical molecular imaging of lymph nodes using a targeted vascular contrast agent. Journal of Biomedical Optics, 10(4), 041205-1–041205-6. https://doi.org/10.1117/1.2007967