| Zugriffsnummer | 20342 |
| Dokumenttyp | Konferenzartikel in Zeitschrift |
| Sprache | Englisch |
| Titel | The PTB microbeam: a versatile instrument for radiobiological research |
| Autor(in); Institution |
Greif, Klaus; 6.4, Ionenbeschleuniger und Referenzstrahlungsfelder, PTB-Braunschweig
Beverung, Wolfgang; 6.4, Ionenbeschleuniger und Referenzstrahlungsfelder, PTB-Braunschweig
Langner, Frank; 6.4, Ionenbeschleuniger und Referenzstrahlungsfelder, PTB-Braunschweig
Frankenberg, Dieter; 6.4, Ionenbeschleuniger und Referenzstrahlungsfelder, PTB-Braunschweig
Gellhaus, Alexandra; University Hospital Essen, Institute of Anatomy, Essen, GERMANY
Banaz Yasar, Ferya; University Hospital Essen, Institute of Anatomy, Essen, GERMANY
|
| Quelle/Jahr | Microdosimetry : proceedings of the 14th International Symposium on Microdosimetry. Radiation Protection Dosimetry: 122 (2006), 1/4, 313 - 315 |
| ISSN | 0144-8420 |
| ISBN | 978-0-19-923171-3 |
| DOI | |
| Verlag | Oxford: Oxford University Press |
| Konferenzangaben | 14th International Symposium on Microdosimetry, Venice, 13-18, November, 2005, Italy |
| Freie Schlagworte | microbeam ; cell irradiation ; immunofluorescence |
| Zusammenfassung | The PTB microbeam is routinely used for the irradiation of living cells using protons (1 - 20 MeV) and alpha particles (1 - 28 MeV). The beam diameter is ≈ 2 μm (fwhm), achieved by focussing, resulting in an excellent energy resolution and practically no scattered particles. Recently, an electrostatic beam scanner was added to the facility which allows targeting of each cell within 1 ms. This and other improvements led to an increase in the experimental speed of the system to a maximum of 50,000 cells per hour including all experimental steps. To improve the versatility of the facility further, a module for automatic quantification of immunocytochemical staining was implemented. This allows the analysis of protein activation, taking into account the positional information of the irradiation run. |
