Zugriffsnummer 18151
Dokumenttyp Zeitschriftenartikel
Sprache Englisch
Titel How to improve quality assurance in fluorometry: fluorescence-inherent sources of error and suited fluorescence standards
Autor(in); Institution
Resch-Genger, Ute; Federal Institute for Materials Research and Testing, Working Group Optical Spectroscopy, Division I.3, BAM, Berlin, GERMANY
Hoffmann, K.; Federal Institute for Materials Research and Testing, Working Group Optical Spectroscopy, Division I.3, BAM, Berlin, GERMANY
Nietfeld, W.; Max Planck Institute for Molecular Genetics, Berlin, GERMANY
Engel, A.; Schott AG, Mainz, GERMANY
Neukammer, Jörg; 8.3, Biomedizinische Optik, PTB-Berlin
Nitschke, R.; Life Imaging Center, Institute of Biology I, Developmental Biology, Freiburg, GERMANY
Ebert, Bernd; 8.3, Biomedizinische Optik, PTB-Berlin
Macdonald, Rainer; 8.3, Biomedizinische Optik, PTB-Berlin
Quelle/Jahr Journal of Fluorescence: 15 (2005), 3, 337 - 362
ISSN 1053-0509
DOI
Verlag Amsterdam [u.a.]: Springer
Freie Schlagworte fluorescence ; standard ; calibration ; microarray ; in vivo imaging ; flow cytometry
Zusammenfassung The scope of this paper is full to illustrate the nee for a improved quality assurance in fluormetry. For this purpose, instrumental sources of error and their influences on the reliability and comparability of fluorescence data are highlighted for frequently used photoluminescence microscopy and flow cytometry to microarry technology as well as in vivo fluorescence imaging. Particularly, the need for and requirements on fluorescence instruments, to enhance the comparability of fluorescence data, and to enable quantitative fluorescence intensity standards.

Zitierung

Resch-Genger, U., Hoffmann, K., Nietfeld, W., Engel, A., Neukammer, J., Nitschke, R., Ebert, B., & Macdonald, R. (2005). How to improve quality assurance in fluorometry: fluorescence-inherent sources of error and suited fluorescence standards. Journal of Fluorescence, 15(3), 337–362. https://doi.org/10.1007/s10895-005-2630-3

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