| Zugriffsnummer | 13013 |
| Dokumenttyp | Zeitschriftenartikel |
| Sprache | Englisch |
| Titel | Macromolecular contrast agents for optical imaging of tumors : comparison of indotricarbocyanine-labeled human serum albumin and transferrin |
| Autor(in); Institution |
Becker, Andreas; Institut für Diagnostikforschung an der Freien Universität Berlin, GERMANY
Riefke, Björn; Institut für Diagnostikforschung an der Freien Universität Berlin, GERMANY
Ebert, Bernd; 8.12, Biomedizinische Optik und NMR-Messtechnik, PTB-Berlin
Sukowski, Uwe; 8.12, Biomedizinische Optik und NMR-Messtechnik, PTB-Berlin
Rinneberg, Herbert; 8, Medizinphysik und metrologische Informationstechnik, PTB-Berlin
Semmler, Wolfhard; Institut für Diagnostikforschung an der Freien Universität Berlin, GERMANY
Licha, Kai; Institut für Diagnostikforschung an der Freien Universität Berlin, GERMANY
|
| Quelle/Jahr | Photochemistry and Photobiology: 72 (2000), 2, 234 - 241 |
| ISSN | 0031-8655 |
| DOI | |
| Verlag | Malden, Mass.: Blackwell Publ. |
| Freie Schlagworte | contrast agents ; fluorescence imaging ; optical imaging ; indotricarbocyanine ; human serum albumin ; transferrin |
| Zusammenfassung | Macromolecules accumulate in solid tumors and can thus be used as carriers for the delivery of attached contrast agents of tumors. We report the synthesis and use of serum protein-dye conjugates consisting of transferrin (Tf) or human serum albumin (HSA) and an indotricarbocyanine (ITCC) derivative as contrast agents for the optical imaging of tumors. The compounds were characterized with respect to their photophysical properties and tested in vitro for their ability to bind to tumor cells and in vivo for their potential to delineate experimental tumors. In contrast to HSA-ITTC, Tf-ITCC showed receptor-mediated uptake by HT29 human colon cancer cells in vitro. After intravenous injection into HT29 tumor-bearing nude mice both compounds induced increased fluorescence contrast of tumors in vivo. After 24 h the contrast between tumor an normal tissue was significantly higher for Tf-ITCC than for HSA-ITTC. Dye-induced fluorescence was found to be predominantly located in perinecrotic areas of the tumor. Furthermore, Tf-ITCC produced fluorescence of viable tumor cells, whereas HSA-ITCC fluorescence was recorded along connective tissue. We conclude that ITCC-labeled Tf and HSA can serve as macromolecular contrast agents for optical imaging of tumors, with Tf-ITCC showing higher efficiency. |
Zitierung
Becker, A., Riefke, B., Ebert, B., Sukowski, U., Rinneberg, H., Semmler, W., & Licha, K. (2000). Macromolecular contrast agents for optical imaging of tumors : comparison of indotricarbocyanine-labeled human serum albumin and transferrin. Photochemistry and Photobiology, 72(2), 234–241. https://doi.org/10.1562/0031-8655(2000)072(0234:mcafoi)2.0.co;2