| Zugriffsnummer | 11054 |
| Dokumenttyp | Zeitschriftenartikel |
| Sprache | Englisch |
| Titel | Evaluation of lesion clustering in irradiated plasmid DNA |
| Autor(in); Institution |
Leloup, C.; Weizmann Institute of Science, Department of Particle Physics, Rehovot, ISRAEL
Garty, G.; Weizmann Institute of Science, Department of Particle Physics, Rehovot, ISRAEL
Assaf, G.; Weizmann Institute of Science, Department of Particle Physics, Rehovot, ISRAEL
Cristovão, A.; Weizmann Institute of Science, Department of Particle Physics, Rehovot, ISRAEL
Breskin, A.; Weizmann Institute of Science, Department of Particle Physics, Rehovot, ISRAEL
Chechik, R.; Weizmann Institute of Science, Department of Particle Physics, Rehovot, ISRAEL
Shchemelinin, S.; Weizmann Institute of Science, Department of Particle Physics, Rehovot, ISRAEL
Paz-Elizur, T.; Weizmann Institute of Science, Department of Biological Chemistry, Rehovot, ISRAEL
Livneh, Z.; Weizmann Institute of Science, Department of Biological Chemistry, Rehovot, ISRAEL
Schulte, R. W.; Loma Linda University Medical Center, Department of Radiation Medicine, Loma Linda, CA, USA
Bashkirov, V.; Loma Linda University Medical Center, Department of Radiation Medicine, Loma Linda, CA, USA
Milligan, J. R.; University of California at San Diego, Department of Radiology, La Jolla, CA, USA
Großwendt, Bernd; 6.6, Grundlagen der Dosimetrie, PTB-Braunschweig
|
| Quelle/Jahr | International Journal of Radiation Biology: 81 (2005), 1, 41 - 54 |
| ISSN | 0955-3002 |
| Verlag | Abingdon: |
| Freie Schlagworte | Radiation biology ; Irradiation plasmid DNA ; DNA strand breaks ; Nanodosimetry |
| Zusammenfassung | Purpose: To measure the yield of DNA strand breaks and clustered lesions in plasmid DNA irradiated with protons, helium nuclei, and γ-rays. Materials and methods: Plasmid DNA was irradiated with 1.03, 19.3 and 249 MeV protons (LET = 25.5, 2.7, and 0.39 keV μm-1 respectively), 26 MeV helium nuclei (25.5 keV μm-1) and γ-rays (137Cs or 60Co) in phosphate buffer containing 2 mM or 200 mM glycerol. Single- and double-strand breaks (SSBs and DSBs) were measured by gel electrophoresis, and clustered lesions containing base lesions were quantified by converting them into irreparable DSBs in transformed bacteria. Results: For protons, SSB yield decreased with increasing LET. The yield of DSBs and all clustered lesions seemed to reach a minimum around 3 keV μm-1. There was a higher yield of SSBs, DSBs and total clustered lesions for protons compared to helium nuclei at 25.5 keV μm-1. A difference in the yields between 137Cs and 60Co γ-rays was also observed, especially for SSBs. Conclusion: In this work we have demonstrated the complex LET dependence of clustered-lesion yields, governed by interplay of the radical recombination and change in track structure. As expected, there was also a significant difference in clustered lesion yields between various radiation fields, having the same or similar LET values, but differing in nanometric track structure. |
Zitierung
Leloup, C., Garty, G., Assaf, G., Cristovão, A., Breskin, A., Chechik, R., Shchemelinin, S., Paz-Elizur, T., Livneh, Z., Schulte, R. W., Bashkirov, V., Milligan, J. R., & Großwendt, B. (2005). Evaluation of lesion clustering in irradiated plasmid DNA. International Journal of Radiation Biology, 81(1), 41–54.